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<!--StartFragment--><p class="MsoNormal" align="center" style="text-align: left;"><b><span style="color:#141314;mso-ansi-language:EN-US">Research Project: </span></b><b><span style="color:#141314;mso-ansi-language:EN-US">Complete Genome
Analysis of <i>Naegleria fowleri</i> and <i>Naegleria lovaniensis</i></span></b></p><p class="MsoNormal" align="center" style="text-align: left;"><b><span style="color:#141314;mso-ansi-language:EN-US">Background</span></b></p><p class="MsoNormal"><i><span style="color:#141314;mso-ansi-language:EN-US">Naegleria</span></i><span style="color:#141314;mso-ansi-language:EN-US"> is a free-living
amoeboflagellate found in freshwater feeding on bacteria. One species, <i>N. fowleri</i>, causes primary amoebic
meningoencephalitis (PAM) in man, a rare but rapidly fatal central nervous
system disease occurring after exposure to contaminated water. <o:p></o:p></span></p><p class="MsoNormal">The pathogenic <i>N.
fowleri</i> is frequently encountered in thermal waters together with its
closest relative, the nonpathogenic <i>N. lovaniensis</i>.<span style="color:#141314"><o:p></o:p></span></p><p class="MsoNormal"><span lang="EN-GB">Sequences
of the internal transcribed spacers (ITS) and the 5.8S ribosomal gene allows to
distinguish eight types of <i>N. fowleri</i>,
which have an uneven distribution in the world. <o:p></o:p></span></p><p class="MsoNormal"><b>Objectives :</b></p><p class="MsoNormal">By comparing the
genome of <i>N. lovaniensis</i> and that of <i>N. fowleri</i>, identify the genes responsible
for the pathogenicity and confirm its probable descent from <i>N. lovaniensis</i>.<o:p></o:p></p><p class="MsoNormal">By comparing the genome
of the three most common <i>N. fowleri</i> types
(<span lang="EN-GB">2, 3 and 5) </span>found in PAM cases, confirm the pathogenicity
genes and identify suitable markers for phylogenetic analyses of <i>N. fowleri</i> strains.<o:p></o:p></p><p class="MsoNormal"><b>Methods :</b></p><p class="MsoNormal">The complete genome sequence
of three strains of <i>N. fowleri</i>
belonging to 3 different types and one strain of <i>N. lovaniensis</i> will be determined using an illumina technology,
before the arrival of the bioinformatician.<o:p></o:p></p><p class="MsoNormal">The bioinformatician
will reconstitute the complete genome of the 4 strains. <o:p></o:p></p><p class="MsoNormal">To assemble the
sequences he/she will be able to rely on the complete genome sequence of <i>Naegleria gruberi</i> (<span lang="EN-GB">41 Mb) </span>available at GenBank. He/she will work at the
Pasteur Institute of Guadeloupe in a scientific environment of young scientists
investigating the molecular epidemiology of <i>Mycobacterium
tuberculosis</i>. He/she will have access to the computers of the Pasteur
Institute in Paris through internet. <o:p></o:p></p><p class="MsoNormal"><b>Terms:</b></p><p class="MsoNormal">The project will last
for two years. The salary will depend on the finances from a grant for a post
doctoral fellowship from the Guadeloupe region. This outcome of the grant will
be known around September 2013. If the grant is accepted, the postdoctoral fellowship
could begin at the end of 2013. The gross salary will be around 32,000 euros.<span lang="EN-GB"><o:p></o:p></span></p><p class="MsoNormal">For further information and for applications contact: </p><div>Dr. Antoine Talarmin</div><div><div>Directeur</div><div>Institut Pasteur de Guadeloupe</div><div>Morne Jolivière</div><div>BP484</div><div>97183 Abymes Cedex</div><div>Guadeloupe France</div><div><a href="mailto:ATALARMIN@pasteur-guadeloupe.fr">ATALARMIN@pasteur-guadeloupe.fr</a></div><div>tel : 05 90 89 69 40</div></div>
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