[Seminars] PSB event reminder

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Calendar Name: seminars
Scheduled for: Monday, March 8 2010, 11:00 - 12:30
Event text:    Dr Stig Uggerhøj Andersen
	       
	       Department of Molecular Biology
	       University of Aarhus
	       
	       Aarhus C
	       DENMARK
Details:       "SHOREmap: simultaneous mapping and mutation
	       identification by deep sequencing"
	       
	       ABSTRACT
	       Identification of causative point mutations after
	       forward genetic mutant screens typically begins with
	       genetic mapping, followed by transformation rescue or
	       candidate gene sequencing. We present a one step
	       alternative: performing hundreds of thousands of
	       genotyping assays while sequencing all candidate genes.
	       This is accomplished by deep sequencing of a pool of F2
	       progeny obtained by crossing to a polymorphic strain and
	       does not require prior knowledge of mapping markers. The
	       application of high-throughput sequencing shortens the
	       overall time required for genetic mapping from months to
	       weeks and, importantly, greatly reduces investigator
	       hands-on time. The steps requiring investigator input
	       are: DNA isolation (1 day), library preparation and
	       validation (4 days), Illumina cluster generation and
	       sequencing (2 days), and data analysis (1 day). Once the
	       mapping population has been established, the present
	       method therefore allows a single investigator to
	       identify a causative mutation within only eight working
	       days – approximately an order of magnitude faster than
	       with conventional methods.

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