[Seminars] PSB event reminder
contact at psb.vib-ugent.be
contact at psb.vib-ugent.be
Tue Feb 8 11:10:01 CET 2011
Calendar Name: seminars
Scheduled for: Thursday, February 10 2011, 11:00 - 12:30
Event text: Dr Renier Van der Hoorn
Chemical Genomics Centre of the Max Planck Society
Max Planck Institute for Plant Breeding Research
Cologne
GERMANY
Details: "Mining the active proteome from plant-pathogen
interactions"
ABSTRACT
The activity of proteins in proteomes and living
organisms can be displayed with Activity-based Protein
Profiling (ABPP). ABPP is a technology that makes use of
small molecule probes that label the active site of
enzyme classes in an activity-dependent manner. Labeling
is covalent and irreversible and facilitates the
separation and detection of labeled proteins using
protein gels and the purification and identification by
mass spectrometry. This method displays functional
proteomic information, which contrasts strongly with
traditional transcriptomic and proteomic datasets, which
are poor predictors on the functional state of
proteins.The introduction and application of ABPP in
plant science is the mission of the Plant Chemetics
laboratory. We have validated and introduced
activity-based probes for papain-like cysteine
proteases, vacuolar processing enzymes, serine
hydrolases, lipases, acyltransferases and the
proteasome. Probes for kinases, glycosidases and other
enzymes are being developed and tested. Procedures for
in vivo labeling and imaging have been established.
Studies of plant-pathogen interactions using these
probes have revealed that the proteasome and Cys
proteases are activated during immune responses, and
suppressed during infection by successful pathogens.
These examples illustrate the use of ABPP to study
protein activities in any organism to address many
different biological questions.
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