[Seminars] PSB event reminder

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Calendar Name: seminars
Scheduled for: Thursday, February 10 2011, 11:00 - 12:30
Event text:    Dr Renier Van der Hoorn
	       
	       Chemical Genomics Centre of the Max Planck Society
	       Max Planck Institute for Plant Breeding Research
	       
	       Cologne
	       GERMANY
Details:       "Mining the active proteome from plant-pathogen
	       interactions"
	       
	       ABSTRACT
	       The activity of proteins in proteomes and living
	       organisms can be displayed with Activity-based Protein
	       Profiling (ABPP). ABPP is a technology that makes use of
	       small molecule probes that label the active site of
	       enzyme classes in an activity-dependent manner. Labeling
	       is covalent and irreversible and facilitates the
	       separation and detection of labeled proteins using
	       protein gels and the purification and identification by
	       mass spectrometry. This method displays functional
	       proteomic information, which contrasts strongly with
	       traditional transcriptomic and proteomic datasets, which
	       are poor predictors on the functional state of
	       proteins.The introduction and application of ABPP in
	       plant science is the mission of the Plant Chemetics
	       laboratory. We have validated and introduced
	       activity-based probes for papain-like cysteine
	       proteases, vacuolar processing enzymes, serine
	       hydrolases, lipases, acyltransferases and the
	       proteasome. Probes for kinases, glycosidases and other
	       enzymes are being developed and tested. Procedures for
	       in vivo labeling and imaging have been established.
	       Studies of plant-pathogen interactions using these
	       probes have revealed that the proteasome and Cys
	       proteases are activated during immune responses, and
	       suppressed during infection by successful pathogens.
	       These examples illustrate the use of ABPP to study
	       protein activities in any organism to address many
	       different biological questions.

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