[Seminars] PSB event reminder
contact at psb.vib-ugent.be
contact at psb.vib-ugent.be
Mon Jan 28 11:10:01 CET 2013
Calendar Name: seminars
Scheduled for: Wednesday, January 30 2013, 11:00 - 12:30
Event text: Dr Jens Boch
Martin Luther University Halle-Wittenberg
Institute of Biology
Department of Genetics
Halle (Saale)
GERMANY
Details: Postdoc Innovation seminar
TALEs and TALENs for targeted genome engineering and
expression control
ABSTRACT
The DNA-binding domain of TALEs (transcription
activator-like effectors) from plant-pathogenic
Xanthomonas bacteria has become an important tool for
the programmable and specific targeting of DNA. Natural
TALEs function as transcription factors in plant cells
to support pathogen colonization of host plants. TALE
proteins bind to DNA via near-identical tandem repeats
of 34 amino acids. Each repeat recognizes one base in
the target DNA sequence via repeat-variable diresidues
(RVDs). The simple and modular repeat architecture
allows rearrangement of TALE repeats to generate
artificial TALEs with virtually any tailored DNA-binding
specificity1. Highly specific genome-editing TALE
nucleases (TALENs) can be engineered for targeted
mutagenesis in plants and a wide variety of other
eukaryotic organisms. We analyzed specificities and
activities of TALEs experimentally in a transient
reporter system using Agrobacterium-mediated expression
in planta. Different RVDs exist in nature, but the
DNA-specificity of only a few of them is known, so far.
We will present novel results on RVD specificity and
efficiency2, as well as the design of programmable gene
switches and programmable precision mutagenesis tools.
TALEs and TALENs are versatile virulence factors for the
pathogen and exceptional tools for biotechnology.
1. Boch, J. et al. Science 326, 1509-1512 (2009).
2. Streubel et al. Nat. Biotechnol. 30, 593-595 (2012).
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