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Calendar Name: seminars
Scheduled for: Tuesday, March 26 2013, 14:00 - 15:30
Event text:    Dr Danièle Werck
	       
	       Institute of Plant Molecular Biology
	       CNRS UPR 2357
	       
	       Strasbourg
	       FRANCE
Details:       “Versatility and fast evolution of the cytochrome P450
	       enzymes in the metabolism of monoterpenols”
	       
	       ABSTRACT
	       The sequencing of plant genomes has revealed that
	       cytochromes P450 (CYPs) form the largest family of plant
	       enzymes in secondary metabolism and that most of their
	       functions were still unknown. This pointed to a very
	       poor understanding of the plant metabolism. We therefore
	       designed complementary strategies to track the
	       overlooked aspects of the secondary metabolism in the
	       model plant Arabidospsis thaliana. One of them is a
	       predictive map of P450 functions in plant metabolism,
	       based on the analysis of gene (co)-expression in
	       publicly available Affymetrix ATH1 microarray data
	       (http://www-ibmp.u-strasbg.fr/~CYPedia/).  Based on
	       CYPedia predictions, a group of co-expressed terpene
	       synthases (TPSs) and CYPs were predicted to be involved
	       in common floral terpenoid metabolism. All four genes
	       were found mainly expressed upon anthesis in the stamen
	       and petal. Investigation of the encodedenzymes in
	       recombinant systems (yeast and Nicotiana benthamiana)
	       and analysis of null and overexpressor mutants revealed
	       new functions of TPS and P450 enzymes in the metabolism
	       of linalool and derived compounds. This work was
	       extended to a more systematic exploration of the family
	       of CYP76 enzymes in Arabidopsis and Catharanthus roseus
	       (in the latter case in connexion with the metabolism of
	       secoiridoïds), which revealed functional versatility,
	       redundancy, specialisation and fast evolution of
	       monoterpenol oxydases, as well as their role in
	       herbicide metabolism.

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