<html><body><div style="font-family: verdana,helvetica,sans-serif; font-size: 9pt; color: #000000"><div><br></div><div>REMINDER</div><div><br></div><hr id="zwchr" data-marker="__DIVIDER__"><div data-marker="__HEADERS__"><b>Van: </b>"Delphine Verspeel" <delphine.verspeel@psb.vib-ugent.be><br><b>Aan: </b>"meetings" <meetings@psb.vib-ugent.be><br><b>Verzonden: </b>Donderdag 3 november 2022 17:01:30<br><b>Onderwerp: </b>You are invited to attend “The endodermal passage cell - novel functions hidden in plain sight?” ON November 24, 2022<br></div><div><br><style>/*<![CDATA[*/p.MsoNormal, li.MsoNormal, div.MsoNormal {
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/*]]>*/</style></div><br><div><div><div><div><div><div><div><div><div><div><div id="zimbraEditorContainer" class="99"><center style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt;">You are receiving this e-mail because you subscribed to our seminars announcements and reminders service at <a href="https://maillist.psb.ugent.be/mailman/listinfo/seminars" rel="nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer nofollow noopener noreferrer" target="_blank">https://maillist.psb.ugent.be/mailman/listinfo/seminars</a>.</center><center><br><span style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt;"> <img data-mce-src="cid:8b0b183ccdc8705b9069736bc3b74ab95fe3b4c9@zimbra" pnsrc="cid:8b0b183ccdc8705b9069736bc3b74ab95fe3b4c9@zimbra" src="cid:8b0b183ccdc8705b9069736bc3b74ab95fe3b4c9@zimbra"></span><h1 style="color: rgb(0, 0, 0); font-family: "segoe ui", "lucida sans", sans-serif; font-size: 9pt; font-style: normal; letter-spacing: normal; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px;"><br></h1><h1 style="text-indent: 0px; background-color: rgb(255, 253, 251);"><i style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt;"><span lang="EN-US"><span style="font-size: 16pt; font-style: normal;">''</span></span></i><i><span lang="EN-US"><span style="font-size: 21.3333px;">The endodermal passage cell - novel functions hidden in plain sight?</span><span style="font-size: 16pt; font-style: normal;">''</span></span></i></h1><h1 style="color: rgb(0, 0, 0); font-family: "segoe ui", "lucida sans", sans-serif; font-size: 9pt; font-style: normal; letter-spacing: normal; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; background-color: rgb(255, 253, 251);"><span style="background-color: rgb(255, 255, 255); font-family: verdana, helvetica, sans-serif; font-size: 9pt;"> </span><br></h1><br><h2 style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt;"><span style="font-size: 24px;">Dr Tonni Grube Andersen</span><br></h2><br><p class="MsoBodyText2" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; margin: 0px;"><br></p><h2 class="MsoBodyText2" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; margin: 0px;"><span style="font-size: 14pt;">Max Planck Institute for Plant Breeding Research, MPIPZ</span><span style="font-family: calibri, sans-serif; font-size: 12pt;"><span style="font-size: 14pt; font-family: verdana, helvetica, sans-serif;"> </span> </span><br></h2><h2 class="MsoBodyText2" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; margin: 0px;"><span style="font-size: 18.6667px;">GERMANY</span></h2><h2 class="MsoBodyText2" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; margin: 0px;"><br></h2><p class="MsoBodyText2" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; margin: 0px;"><br></p><br><br><h2 style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt;"><span style="font-size: 16pt;">Thursday, November 24, 2022</span><br><span style="font-size: 16pt;">11:00</span></h2><p class="MsoNormal" align="center" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; text-align: center; margin: 0px;"><span lang="EN-US" style="font-size: 16pt; font-family: calibri, sans-serif;"> </span></p><p class="MsoNormal" align="center" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; text-align: center; margin: 0px;"><span lang="EN-US" style="font-size: 16pt; font-family: calibri, sans-serif;"> </span></p><p class="MsoNormal" align="center" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; text-align: center; margin: 0px;"><span lang="EN-US" style="font-size: 16pt; font-family: calibri, sans-serif;"><strong> </strong></span><br></p><p class="MsoNormal" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; text-align: justify; margin: 0px;"><br></p><p class="MsoNormal" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; text-align: justify; margin: 0px;"><br></p><p class="MsoNormal" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; text-align: justify; margin: 0px;"><br></p><p class="MsoNormal" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; text-align: justify; margin: 0px;"><br></p><p class="MsoNormal" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; text-align: justify; margin: 0px;"><br></p><p class="xmsonormal" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; margin: 0px; text-align: justify; background: rgb(255, 253, 251);"><br></p><p class="MsoNormal" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; margin: 0px; text-align: justify;"> </p><p class="MsoNormal" style="margin: 0px; text-align: justify; line-height: 150%;"><span lang="EN-US" style="font-family: verdana, helvetica, sans-serif; font-size: 10pt;">Anatomically, roots consist of the central vascular cylinder enclosed by the endodermal, cortex and epidermal cell layers. The innermost endodermis contains apoplasmic barriers - Casparian Strips (CS) - that allow selective solute uptake from the soil by forcing transport across the plasma membrane. In older root parts, the entire surface of most endodermal cells is covered by suberin lamellae, which block transport across the plasma membrane and thereby likely renders the endodermal cell insensitive to its surroundings. However, some few cells situated at the endodermal xylem poles remain unsuberized. As they may form a path of low resistance for radial flow of solutes through an otherwise sealed endodermis, these cells have been termed “passage cells” (PCs) despite lack of functional evidence. Intriguingly, suberization and passage cell occurrence is highly dynamic and responsive to nutritional stress, which suggests an active role in nutrient homeostasis. </span></p><p class="MsoNormal" style="margin: 0px; text-align: justify; line-height: 150%;"><span lang="EN-US" style="font-family: arial, sans-serif;"><span style="font-family: verdana, helvetica, sans-serif; font-size: 10pt;">While the effect of several nutrients has been investigated, very little is known of how the N-status affects this intriguing dynamic barrier system. Surprisingly, we found that nitrogen starvation results in an oversuberization of the endodermis and consequent closure of PCs. . To get mechanistic insight into this, we employed tissue-specific Translating Ribosome Affinity Purification (TRAP) which allowed us to gain new insights into how old root parts respond to chronic N-starvation a tissue-specific manner. We use fluorescent, transcriptional reporter lines and vertical microscopy to investigate long-term responses of candidate genes upon varying mineral nitrogen availability. Our findings reveal exciting new insights into the role of this intriguing cell type. As tissue-specific roles in nitrogen use efficiency (NUE) have a huge agricultural impact, these finding may allow us to expand on our current models for NUE to include older root parts and their role in (a)biotic communication. </span><i><span style="font-family: verdana, helvetica, sans-serif; font-size: 10pt;"> </span></i></span></p><p class="MsoNormal" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; margin: 0px; text-align: justify;"> <br></p><p class="MsoNormal" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; margin: 0px; text-align: justify;"><span lang="EN-US" style="font-size: 11pt; font-family: arial, sans-serif;"> </span><span lang="EN-US" style="font-size: 11pt; font-family: helvetica, sans-serif; color: black;"> </span><span lang="EN-US" style="font-size: 11pt; font-family: calibri, sans-serif; color: black;"></span></p><p class="MsoNormal" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; text-align: justify; margin: 0px;"><br></p><p class="MsoNormal" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; text-align: justify; margin: 0px;"><br></p><p class="MsoNormal" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; text-align: justify; margin: 0px;"><br></p><p class="MsoNormal" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; text-align: justify; margin: 0px;"><br></p><p class="MsoNormal" style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt; text-align: justify; margin: 0px;"><br></p><h3 style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt;">Jozef Schell seminar room<br>Technologiepark 71 - 9052 </h3><br><br><br><br><span style="color: rgb(0, 0, 0); font-family: verdana, helvetica, sans-serif; font-size: 9pt;">Invited by Prof Dirk Inzé and Prof Bert De Rybel</span></center><center style="color: rgb(0, 0, 0); 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