The BiGCaT Bioinformatics group at Maastricht University is happy to announce the release of PathVisio 2.0. [1] What is PathVisio? PathVisio is a tool for creating and analysing biological pathway diagrams. Stay organized by using PathVisio as a notebook to collect the various bits of information related to a biological research subject. Create images suitable for presentation or publication. Draw pathways, export them to many image formats, annotate them with links to online biological databases such as Ensembl or Entrez gene, and add comments and literature references from pubmed. PathVisio is fully compatible with WikiPathways [2], a wiki where researchers can contribute pathway knowledge. What is new? New in PathVisio 2.0 is the ability to import experimental datasets and visualize them on top of pathways. Explore large datasets in a way that is more interesting and understandable than just a huge spreadsheet. Import microarray, proteomics or metabolomics data. Microarray reporters will be automatically converted to gene or protein identifiers. You can customize the visualization, using gradients, boolean color rules, or colored icons. Perform over-representation analysis to find the pathway that was most affected by experimental conditions. We prepared a Visual Tour [3] to get an overview of new and old features. For Developers: PathVisio has a plugin interface that lets users customize it to new analysis types, new visualization methods and new pathway formats. PathVisio is fully open source, and we're always looking for Java developers who are interested in contributing, either to new plugins or to the core of the program. Contact us on our mailinglist [4]. [1] http://www.pathvisio.org/ [2] http://www.wikipathways.org/ [3] http://www.pathvisio.org/wiki/VisualTour [4] http://www.pathvisio.org/wiki/MailingLists