Course Reminder Applications are OPEN until May 7th 2013 Early decisions available. *TA-AFADM13* Transcriptome Assembly, Automatic Annotation and Data Mining with Ana Conesa and Fernando Garcia IMPORTANT DATES for TA-AFADM13 Deadline for applications: May 7th 2013 Notification of acceptance dates: EARLY: Apr 19th 2013 (on special request, see Application) NORMAL: May 8th 2013 Course date: May 20th - May 23rd 2013 http://gtpb.igc.gulbenkian.pt/bicourses/TA-AFADM13 This course is particularly suitable for those seeking training in using NGS data for tanscriptomics (RNA-seq), and in extensively annotating with Blast2GO, the most popular and powerful tool for this purpose. Course description: This is a combined course in RNA-seq and functional annotation aimed at scientists working in next generation sequencing transcriptomics who want to extract the best possible information from their data. The first part of the course is a practical tutorial on Blast2GO, the most popular framework for functional annotation of sequences. Participants will also learn how to generate de novo functional labels, such as GO terms and KEGG pathways, for their sequence data and how to extract relevant information from these annotations, i.e. visualization and enrichment analysis using the Gene Ontology. The second part of the course covers many relevant aspects of RNA-seq analysis, form quality control, to mapping, reconstruction of transcripts and differential expression. Participants will learn how to deal with RNAseq data both in the presence and absence of a reference genome. At the end of the course, participants can expect to have acquired skills to use multiple software tools to analise RNA-seq data and use Blast2GO. The course will be based on the use of Blast2GO application and will mainly comprise exercises and practical cases. Participants may bring their own data. *Who should attend?* Experimentalists and bioinformaticians working on EST, NextGeneration Sequencing and microarray design projects, specially (but not exclusively) of non-model species. No programming knowledge is required. *Course Pre-requisites:* Basic knowledge in Molecular Biology and Statistics. Pedro Fernandes GTPB Coordinator -- Pedro Fernandes Instituto Gulbenkian de Ciência Apartado 14 2781-901 OEIRAS Tel +351 21 4407912 http://gtpb.igc.gulbenkian.pt _______________________________________________ Gtpb mailing list Gtpb@igc.gulbenkian.pt https://lists.igc.gulbenkian.pt/mailman/listinfo/gtpb