Transcript identification and quantification through RNA sequencing is a key tool throughout the life sciences. With so many workflows now available, making a start analysing your own RNA-seq data can be a bewildering experience. Let our expert bioinfomaticians guide you through a tried-and-tested RNA-seq data analysis workflow at the country's leading open-access genome facility. Our ever-popular 'Introduction to RNA-seq Data Analysis' workshop will be running again this spring on the 9-10th May 2019 at the Kings Buildings campus, University of Edinburgh. This course covers the following topics: * Introduction to Next Generation Sequencing * Quality control and data pre-processing (FastQC, cutadapt) * Mapping to a reference genome (STAR, SAMtools) * Visualisation of mapped reads (SAMtools, IGV) * Introduction to R (R, Rstudio) * Estimating gene count (featureCounts) * Differential expression analysis (R, RStudio, edgeR, rtracklayer, ggplot2, pheatmap) * Functional analysis (GSEABase) The two day course costs £400 including lunches and refreshments. For more information see our training website at http://genomics.ed.ac.uk/services/introduction-rna-seq-data-analysis or to sign up fill out this form at https://www.surveymonkey.co.uk/r/736PQLC If you have any further questions about the course or any others that we provide please drop us a line at edge-training@ed.ac.uk<mailto:edge-training@ed.ac.uk> Not the course your looking for? Tell us what training courses you need in this short survey https://www.surveymonkey.co.uk/r/7M6QB2D Kind Regards Nathan Medd Training and Outreach Manager Edinburgh Genomics' Privacy Notice can be viewed at: http://genomics.ed.ac.uk/about-us/privacy-notice This email and any attachments are confidential and intended solely for the use of the recipient(s) to whom they are addressed. If you have received it in error, please destroy all copies and inform the sender. The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336.