BioSB course Protein Structures: production, prowess, power, promises, and problems (4th edition) Date: 31 October - 3 November 2017 Location: CMBI, Radboudumc, Nijmegen, the Netherlands Course coordinator: Gert Vriend (CMBI, Radboudumc) Lecturers: Daniel Hoffmann (University Duisburg/Essen (TBC)), Hanka Venselaar (CMBI), Wouter Touw (CMBI) *Website*: course website <http://biosb.nl/education/course-portfolio/protein-structures-production-prowess-power-promises-and-problems/> *Registration:* registration form <http://biosb.nl/education/course-portfolio/protein-structures-production-prowess-power-promises-and-problems/enrollment-protein-structures-course/> Target audience The course is suitable for both PhD students working in structural bioinformatics as well as for PhD students in the life sciences who want to learn more about using structural informatioin in their research. The course schedule accommodates both groups, parallel sessions for both groups will be scheduled when necessary. Course Description In the right hands, protein structures are a ‘power’ful tool to answer bio-molecular questions. Knowledge of the structure is a pre-requisite for rational drug design, for biotechnology, for chemical biology, and for answering a whole series of biomedical questions. In this course we will discuss the ‘production’ of protein structures by NMR, X-ray, and homology modelling. These methods all have their pro’s and cons so a certain ‘prowess’ is needed to follow all ‘promises’ and start attacking bio-medical ‘problems’ using protein structures. The course will be split in three parts: - Part 1) Looking and seeing things in protein structures, learning to operate the software, understanding some of the algorithms. - Part 2) Protein structure determination (prediction) with NMR, X-ray, and homology modelling, and the possibilities and problems that come with each of these three techniques. - Part 3) Applying all that was learned in real-life example studies. Course topics Day 1: Homology modelling seminar Day 2: Protein structure comparison seminars Day 3: Electrostatics and molecular dynamics seminars Day 4: Protein structure determination and validation seminars Day 5: Own project executed with help from CMBI staff Examples of questions you will be able to answer after follwing the course: - Why is a person with this mutation sick? Or in other words, how does the molecular phenotype cause a disease state? - This enzyme converts mannose. Can I make is specificity broader? - This enzyme doesn’t function in my in vitro assay. Should I add some ions? - I want to add a tag to my enzyme, should I put it on the N-terminus, the C-terminus, or is something different needed? - This receptor binds a ligand, but if I look at the structure, that ligand doesn’t fit at all. Can I predict motions that take place upon ligand binding? This course is part of the Education Programme of BioSB <http://biosb.nl/education/course-portfolio-2/>, the Netherlands Bioinformatics and Systems Biology Research School, which offers training and education for in bioinformatics and systems biology. More information about BioSB can be found at www.biosb.nl. ** ** *Femke Francissen* Community manager BioSB Research School *NEW V*isiting address (as of 1 July 2017): Jaarbeurs Innovation Mile (JIM) | 6th floor Beatrixgebouw | Jaarbeursplein 6 | 3521 AL Utrecht *NEW **Postal address *(as of 1 July 2017): Postbus 8500 | 3503 RM Utrecht E-mail: femke.francissen@biosb.nl Mobile: +31 6 17 90 4888 Skype: femke.francissen Website: www.biosb.nl LinkedIn link <http://nl.linkedin.com/in/femkefrancissen>