Target audience
Course Description
In
the right hands, protein structures are a 'power'ful tool to answer
bio-molecular questions. Knowledge of the structure is a pre-requisite
for
rational drug design, for biotechnology, for chemical biology, and for
answering a whole series of biomedical questions. In this course we
will
discuss the 'production' of protein structures by NMR, X-ray, and
homology
modelling. These methods all have their pro's and cons so a certain
'prowess'
is needed to follow all 'promises' and start attacking bio-medical
'problems' using
protein structures.
The
course will be split in three parts.
Part
1) Looking and seeing things in protein structures, learning to operate
the
software, understanding some of the algorithms.
Part
2) Protein structure determination (prediction) with NMR, X-ray, and
homology
modelling, and the possibilities and problems that come with each of
these
three techniques.
Part
3) Applying all that was learned in real-life example studies.
Course topics
Day 1: Homology modelling
Day 2: Protein structure determination and validation
Day 3: Electrostatics and molecular dynamics
Day 4: Protein structure comparison
Day 5: Own project executed with
help from CMBI staff.
Examples
of questions you will be able to answer after follwing the course:
1)
Why is a person with this mutation sick?
Or in other words, how does the molecular phenotype cause a disease
state?
2)
This enzyme converts mannose. Can I make
is specificity broader?
3)
This enzyme doesn't function in my in
vitro assay. Should I add some ions?
4)
I want to add a tag to my enzyme, should
I put it on the N-terminus, the C-terminus, or is something different
needed?
5)
This receptor binds a ligand, but if I
look at the structure, that ligand doesn't fit at all. Can I predict
motions
that take place upon ligand binding?
More
information about the course programme can be found on:
http://www.nbic.nl/education/nbic-phd-school/course-schedule/protein-structures-production-prowess-power-promises-and-problems/.
NBIC PhD School
This
course is part of the Education Programme of the NBIC PhD School. The
NBIC PhD
School offers training and education for PhD students in
bioinformatics.
More
information about the NBIC PhD School can also be found on
http://www.nbic.nl =>
Education => NBIC PhD School.
More information
For
more information about the course you can contact Celia van Gelder
(NBIC
Education) at celia.van.gelder@nbic.nl.
-- Dr. Celia W.G. van Gelder Coordinator Education CMBI Projectleader Education NBIC tel. +31-(0)24-3666120 e-mail: C.vanGelder@cmbi.ru.nl websites: www.cmbi.ru.nl, www.nbic.nl Postal address: CMBI 260 Radboud University Nijmegen-Medical Centre /NCMLS PO Box 9101, 6500 HB Nijmegen The Netherlands