BioSB RNA-seq data analysis course (7th edition) Date: 6-8 November 2017 Location: Leiden University Medical Center, the Netherlands Course coordinators: Peter-Bram ‘t Hoen (LUMC), Leon Mei (LUMC), Jan Oosting (LUMC), Szymon Kielbasa (LUMC) Course website: http://biosb.nl/education/course-portfolio/rna-seq/ . Registration: http://biosb.nl/education/course-portfolio/rna-seq/ enrollment-rna-seq-2017/ Course description The BioSB research school and partner LUMC are organizing a 3-day course on RNA-seq data analysis. This is an advanced course for people with experience in NGS. The course will consist of lectures, Galaxy and R practicals and will cover the analysis pipelines for differential transcript expression and variant calling. Examples will be taken from human and mouse studies. The course does not cover prokaryotic RNA profiling nor plant- and metagenomics aspects. Course topics - RNA-seq experimental approaches and study design - Quality control and alignment - Statistics for differential gene expression - eQTL analysis and allele specific expression - Single cell RNA sequencing - Fusion transcript detection - Small RNA profiling - Software for RNA-seq data analysis Target audience Participants for the RNA-seq course should preferably have participated in the general NGS course or otherwise have demonstrated hands-on experience with NGS data analysis. The course is aimed at PhD students and post-docs, but scientific programmers and data analysts with a background in biology and bioinformatics may also attend. NB: The practicals are mostly in R. We require that participants have at least basic knowledge of R and experience in running R scripts. Participants must bring own laptops capable of running RStudio. Please download and install RStudio before the course. For Galaxy tutorial, please install firefox or google chrome web browser. Registrations will be handled on a first-come-first-served basis but experience and motivation will be reviewed.Registrations will be handled on a first-come-first-served basis but experience and motivation will be reviewed. Seats have been reserved for participants in the FP7-funded projects RD-Connect <http://rd-connect.eu/> and BBMRI-NL <https://www.bbmri.nl/>. This course is part of the Education Programme of BioSB <http://biosb.nl/education/course-portfolio-2/>, the Netherlands Bioinformatics and Systems Biology Research School, which offers training and education for in bioinformatics and systems biology. More information about BioSB can be found at www.biosb.nl. *​* *​* *Femke Francissen* Community manager BioSB Research School *NEW V*isiting address (as of 1 July 2017): Jaarbeurs Innovation Mile (JIM) | 6th floor Beatrixgebouw | Jaarbeursplein 6 | 3521 AL Utrecht *NEW **Postal address *(as of 1 July 2017): Postbus 8500 | 3503 RM Utrecht E-mail: femke.francissen@biosb.nl Mobile: +31 6 17 90 4888 Skype: femke.francissen Website: www.biosb.nl LinkedIn link <http://nl.linkedin.com/in/femkefrancissen>