Dear Severin, The best/easiest thing to do then is to take a gene that matches the EST (eg. Ot02g00220, in this case) do a blast with it against the EST sets and from the resulting hits select the sequence(s) to download. I hope this helps but don't hesitate to contact me again it not. best regards, lieven ----- Oorspronkelijk bericht -----

Van: "Severin Sasso" <severin.sasso@uni-jena.de> Aan: "lieven sterck" <lieven.sterck@psb.ugent.be> Verzonden: Maandag 28 november 2011 11:02:45 Onderwerp: Ostreococcus EST sequence

Dear Lieven,

I have found the following EST for Ostreococcus tauri on BOGAS, but I could not find a way to download the nucleotide sequence (and for some reason, the EST information on the JGI browser seems different). Would it be possible to get the sequence information for this EST?

Gene ID: CH1_PKS3 Assembly: chrom02 Position: 42529..46799

Thank you for your help! Severin

-- Prof. Severin Sasso Junior Professor of Molecular Botany Friedrich Schiller University Institute of General Botany and Plant Physiology Dornburger Str. 159 D-07743 Jena, Germany

Tel. +49 (0)3641 949458 (office) Tel. +49 (0)3641 949246 (lab) Fax +49 (0)3641 949232 http://www.uni-jena.de/en/molecular_botany.html

-- ============================================================== Lieven Sterck, PhD Tel:+32 (0)9 3313821 Fax:+32 (0)9 3313809 VIB Department of Plant Systems Biology, UGent Bioinformatics and Evolutionary Genomics Division Technologiepark 927, B-9052 Gent, Belgium Email: lieven.sterck@psb.vib-ugent.be Website: http://bioinformatics.psb.ugent.be ============================================================== "Facts are meaningless. You could use facts to prove anything that's even remotely true" - H. Simpson