[Beg-bogas] Pichia pastoris blast

Dear addressee, I was blasting some Pichia pastoris (GS115) sequences in your pages (http://bioinformatics.psb.ugent.be/blast/moderated/?project=bogas_Picpa) and keep getting the following error when trying to retrieve sequences: sh: FN392322.1: command not found [fastacmd] ERROR: Entry "emb" not found Use of uninitialized value in pattern match (m//) at /www/group/biocomp/extra/cgi-bin/biocomp/blast/get_fasta.pl line 53. Use of uninitialized value in concatenation (.) or string at /www/group/biocomp/extra/cgi-bin/biocomp/blast/get_fasta.pl line 56. Use of uninitialized value in concatenation (.) or string at /www/group/biocomp/extra/cgi-bin/biocomp/blast/get_fasta.pl line 59. Use of uninitialized value in concatenation (.) or string at /www/group/biocomp/extra/cgi-bin/biocomp/blast/get_fasta.pl line 62. Am I doing something wrong? I also remember blasting earlier to "large contigs", now I can only find whole genomes to choose... do I remember wrong or has something been changed lately? Best Regards, Laura Näätsaari -- Laura Näätsaari Institute of Molecular Biotechnology, Graz University of Technology Petersgasse 14/II 8010 Graz AUSTRIA Phone: +43 316 873 4077 Fax: +43 316 873 4071 Mail: naeaetsaari@student.tugraz.at

Hi, the problem should be fixed now. Fastacmd seemed to be a bit clueless what to do with seq id's that had |-signs in them. Thx for reporting this! Kenny On Thu, 6 Aug 2009, Laura Naeaetsaari wrote:
Dear addressee,
I was blasting some Pichia pastoris (GS115) sequences in your pages (http://bioinformatics.psb.ugent.be/blast/moderated/?project=bogas_Picpa) and keep getting the following error when trying to retrieve sequences: sh: FN392322.1: command not found [fastacmd] ERROR: Entry "emb" not found Use of uninitialized value in pattern match (m//) at /www/group/biocomp/extra/cgi-bin/biocomp/blast/get_fasta.pl line 53. Use of uninitialized value in concatenation (.) or string at /www/group/biocomp/extra/cgi-bin/biocomp/blast/get_fasta.pl line 56. Use of uninitialized value in concatenation (.) or string at /www/group/biocomp/extra/cgi-bin/biocomp/blast/get_fasta.pl line 59. Use of uninitialized value in concatenation (.) or string at /www/group/biocomp/extra/cgi-bin/biocomp/blast/get_fasta.pl line 62. Am I doing something wrong? I also remember blasting earlier to "large contigs", now I can only find whole genomes to choose... do I remember wrong or has something been changed lately? Best Regards, Laura Näätsaari -- ================================================================== Kenny Billiau Web Developer Tel:+32 (0)9 331 36 95 fax:+32 (0)9 3313809 VIB Department of Plant Systems Biology, Ghent University Technologiepark 927, 9052 Gent, BELGIUM kenny.billiau@ugent.be http://bioinformatics.psb.ugent.be ================================================================== "What we feel isn't important. The only question is what we do." -- Rohl
participants (2)
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Kenny Billiau
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Laura Naeaetsaari