http://www.ncbi.nlm.nih.gov/pubmed/19439512?dopt=Abstract
Evolution of gene function and regulatory control after whole-genome
duplication: Comparative analyses in vertebrates.
Kassahn KS, Dang VT, Wilkins SJ, Perkins AC, Ragan MA.
Genome Res. 2009 May 13.
The significance of whole-genome duplications (WGD) for vertebrate
evolution remains controversial, in part because the mechanisms by
which WGD contributed to functional evolution or speciation are still
incompletely characterised. Fish genomes provide an ideal
context in which to examine the consequences of WGD, because the
teleost lineage experienced an additional WGD soon after divergence
from tetrapods and five teleost genomes are available for comparative
analysis. Here we present an integrated approach to characterise
these post-duplication genomes based on genome-scale synteny,
phylogenetic, temporal and spatial gene expression, and protein
sequence data. A minimum of 3-4% of protein-coding loci have been
retained in two copies in each of the five fish genomes and many of
these duplicates are key developmental genes that function as
transcription factors or signalling molecules. Almost all duplicate
gene pairs we examined have diverged in spatial and/or temporal
expression during embryogenesis. A quarter of duplicate pairs have
diverged in function via the acquisition of novel protein domains or
via changes in the subcellular localisation of their encoded proteins.
We compared the spatial expression and protein domain architecture of
zebrafish WGD-duplicates to those of their single mouse ortholog and
found many examples supporting a model of neofunctionalisation.
WGD-duplicates have acquired novel protein domains more often than have
single-copy genes. Post-WGD changes at the gene regulatory level were
more common than changes at the protein level. We conclude that the
most significant consequence of WGD for vertebrate evolution has been
to enable more-specialised regulatory control of development via the
acquisition of novel spatio-temporal expression domains. We find
limited evidence that reciprocal gene loss led to reproductive
isolation and speciation in this lineage.
Is it possible to save the PDF on the Article drive? Thanks, Klaas
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Klaas Vandepoele, PhD
Tel. 32 (0)9 33 13822
VIB Department of Plant Systems Biology, Ghent University
Technologiepark 927, 9052 Gent, Belgium
E-mail: Klaas.Vandepoele@psb.vib-ugent.be
Website: http://bioinformatics.psb.ugent.be/
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it's hard to keep the animo from driftin' away nowadays