-------- Original Message -------- Subject: Re: Apple full transcripts array Date: Fri, 9 Jul 2010 13:51:54 +0200 From: Jean-Pierre Renou <jeprenou@angers.inra.fr> To: Yves Van de Peer <yves.vandepeer@psb.vib-ugent.be> CC: jean-pierre renou <renou@evry.inra.fr>, rogerb@u.washington.edu, riccardo.velasco@iasma.it, Jean-Marc Celton <jcelton@mail.biotech.uwc.ac.za>, zharkikh@myriad.com, michael.egholm@roche.com, sgardiner@hortresearch.co.nz, Charles Eric Durel <Charles-Eric.Durel@angers.inra.fr>, laurens <francois.laurens@angers.inra.fr>, Elisabeth Chevreau <chevreau@angers.inra.fr>, bogden@genomex.com, yves.vandepeer@psb.ugent.be, ananth@eecs.wsu.edu, rhellens@hortresearch.co.nz, stefano.toppo@unipd.it, martin.kater@unimi.it, alessandra.stella@tecnoparco.org, durel <durel@angers.inra.fr> Dear Yves For sure I will keep you informed, it is obvious that we can make significant benefits by joining our efforts and expertise to make sense of the data. Currently I am just beginning to work on apple in Angers, so I need few months to set up the probe design. My plan is to produce numerous apple transcriptome data with the chips in 2011 on dozens of samples. If the other apple-scientists plan to use the same array for their experiments and are interested to share the results, we can expect to begin meta-analyses quite soon. I will do my best to be as quick as possible, we do have numerous samples in the -80°C already waiting for us. Best regards JP Le 9 juil. 2010 à 08:58, Yves Van de Peer a écrit :
Dear all,
For what it's worth, we would be highly interested in having a look at such transcriptome data for apple, with the aim to reconstruct transcriptional regulatory networks. Recently, we have developed sophisticated software to identify regulators such as transcription factors, signal transducers, but also microRNAs, based on transcriptome and other data. Please keep us in the loop if you think we might be able to help or if you'd like to collaborate on such a 'transcriptome' project.
Best regards,
Yves
Dear colleague Charles-Eric Durel informed me that you expect to have very soon an apple microarray based on the genome sequence. Actually I am deeply involved in functional genomics and transcriptomics since numerous years (http://www.versailles.inra.fr/urgv/renou.htm) and I will join the "Genhort" laboratory of Angers, to work on apple, next month. Indeed we had exactly the same feeling as you, and I got financial support from INRA to design a full transcript microarray based on on the genome sequence very soon. We totally agree with you that such a powerful tool should be available for the whole apple community without restriction. Moreover we all know that we can expect much more power in the future meta-analyse we could plan if we avoid to multiply the platforms, the homogeneity of the data being a guarantee of quality. We think that, regarding to high throughput sequencing methods which are supposed to be more powerful in terms of gene discovery (yet not so sure for transcript quantification), the major interest of a microarray remains its low price. This would provide the opportunity to analyze hundreds of samples such as many biological replicates, time course experiments, various types of samples etc etc...... The higher is the quantity of analysed samples, the higher is the value of a transcriptomic resource! For this reasons, after having considered all the possibilites, we are most in favour of the "Maskless Arrays Synthetiser" technology which provides very cheap, powerful and evolutive chips. Currently we have very good experiences with the new multiplex Nimblegen chips (12 X 120.000 probes) which would provide full transcript arrays for 85€ per unit. Agilent chips are good too and use the same technology but are more expensive and provide less probes per unit. The same for Illumina (which are rather cheap). Affymetrix chips are definetely expensive and not evolutive, due to the Mask technology. The bioinformatic team of my current lab has developed an algorithm (derived from SPDAS, Thareau et al. 2003) to design very specific oligoprobes that we plan to use, because Nimblegen doesn't have such skills (at least it's that they told us). François Laurens and I will meet Ricardo and his staff next July to discuss all these points and to optimize our collaboration. If you are interested by this initiative we would be happy to share this resource with you and all the apple community. I am pretty sure that we can all make a very good job altogether and make fast progress in terms of deciphering apple gene regulation networks, thanks to the highly valuable genetic resources already available. I think that it would be a nice opportunity to meet altogether during the next Rosacae Symposium in SA. Maybe we could organize a workshop? Best Regards Jean Pierre
-- Yves Van de Peer, PhD.
Professor in Bioinformatics and Genome Biology Associate Department Director, VIB Department of Plant Systems Biology Group Leader Bioinformatics and Systems Biology Ghent University Technologiepark 927 B-9052 Ghent Belgium
Phone: +32 (0)9 331 3807 Cell Phone: +32 (0)476 560 091 Fax: +32 (0)9 331 3809 email: yves.vandepeer@psb.vib-ugent.be