'total loss' vrees ik ... ;-)
-------- Original Message --------
Dear all,
I'm looking for away to measure the density of a biopolymer
lighter than 0.00001g.
I could of course collect enough of this polymer to get a mass
> 0.0001g, and submerge it then in water to measure the volume via
water displacement...
But then I would need a LOT of material. And given it is not
necessarily easy to get the pure material in large quantities, any
other way would be welcome. One option could be a CsCl column and
centrifuge it with the biopolymer as was done some time ago to purify
DNA
The problem there is that I have no way of labeling the polymer to
be able to see where in the column the polymer ends up and as I will
need a density ladder, the measurement will remain approximative.
Alternatively, if you know a (UGent/VIB) department that has a
machine that does it all and is sensitive enough, let me know.
best regards
Stephane
--
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Lieven Sterck, PhD
Tel:+32 (0)9 3313821 Fax:+32 (0)9 3313809
VIB Department of Plant Systems Biology, UGent
Bioinformatics and Evolutionary Genomics Division
Technologiepark 927, B-9052 Gent, Belgium
Email: lieven.sterck@psb.vib-ugent.be
Website: http://bioinformatics.psb.ugent.be
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"You tried your best and you failed miserably.
The lesson is: never try!" - H. Simpson