November 2016 - confocal@psb.ugent.be

[Confocal] test3
by Evelien Mylle 29 Nov '16

29 Nov '16

Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB Department of Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 www.psb.ugent.be

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[Confocal] test2
by Evelien Mylle 29 Nov '16

29 Nov '16

Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB Department of Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 www.psb.ugent.be

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[Confocal] Fwd: Zeiss exciter issues: laser and fluorescence lamp
by Evelien Mylle 29 Nov '16

29 Nov '16

Dear all, A new argon laser has been installed yesterday for the Zeiss Exciter. The fluorescence lamp was already OK since some weeks. Enjoy imaging. In case of any problems, don't hesitate to contact Daniel or me. Greetings, Evelien > From: "Evelien Mylle" <evmyl(a)psb.vib-ugent.be> > To: "Evelien Mylle" <evmyl(a)psb.vib-ugent.be> > Sent: Wednesday, October 19, 2016 1:31:16 PM > Subject: Fwd: Zeiss exciter issues: laser and fluorescence lamp >> From: "Evelien Mylle" <evmyl(a)psb.ugent.be> >> To: confocal(a)psb.ugent.be >> Sent: Wednesday, October 19, 2016 1:31:02 PM >> Subject: Fwd: Zeiss exciter issues: laser and fluorescence lamp >>> Dear all, >>> I noticed that the argon laser is not working optimal anymore . The plasm is >>> almost degrades, which makes it unusable at the normal output (= position of >>> the small wheel just before the green light turns into the red light). >>> This wheel is now at a position that gives the best output. (approx around 4,7 >>> A, whereas normally this is around 6A). Please don't change, I put a tape >>> around it. Above this position there is no output at all. (you should see a >>> purple light, not an orange light). >>> A new laser is ordered and will be installed. >>> Also the lamp of the fluorescence lamp has been changed . This lamp was already >>> a lot above this maximum lifetime and caused a lot of noise. Also the lamp >>> sometimes felt out during the experiment. >>> Dued to a broken collector glas inside this lamphouse, it was impossible to make >>> a proper alignment with the new lamp. The Zeiss person will look into this >>> tomorrow to try to get it as good as possible for the moment until the part is >>> repaired. >>> I would like to address once more to contact Daniel or me whenever you encounter >>> some problems. >>> Thanks in advance, >>> Greetings, >>> Evelien >>> Evelien Mylle - Lab Manager / Technician >>> Advanced Live Cell Imaging >>> VIB Department of Plant Systems Biology >>> Ghent University >>> Technologiepark 927 - 9052 Ghent - Belgium >>> Tel. +32(0)9 331 39 34 >>> www.psb.ugent.be

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[Confocal] test
by Evelien Mylle 29 Nov '16

29 Nov '16

Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB Department of Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 www.psb.ugent.be

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[Confocal] spinning disc filter wheel error
by Daniel Van Damme 26 Nov '16

26 Nov '16

Dear all, The filter at pos 10 of the spinning disc got loose and I had to remove it. The system runs, but currently you cannot use pos10 at all (there is nothing in there now). Also, when using the dual camera, rigorously check the calibration as I had to remove the camera to get to the filter wheel. Have fun imaging, d Daniel Van Damme - Group leader Advanced Live Cell Imaging VIB Department of Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 13 www.psb.ugent.be

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[Confocal] FV10 ASW confocal free, Joanna Boruc is not here and she forgot to unreserved
by Roman HUDECEK 24 Nov '16

24 Nov '16

Roman HUDECEK - Predoctoral fellow Programmed Cell Death VIB Department of Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 16 www.psb.ugent.be

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[Confocal] CLSM 710 will be free this afternoon from 3pm till 5pm. Feel free to use it!
by Tom Van Hautegem 24 Nov '16

24 Nov '16

Enjoy! Cheers, Tom Tom Van Hautegem - Postdoctoral fellow Systems Biology Of Yield VIB Department of Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 35 29 www.psb.ugent.be

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[Confocal] 710 free until 14.00
by Ana Fernandez 14 Nov '16

14 Nov '16

Ana Fernandez - Postdoctoral fellow Root Development VIB Department of Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 38 44 www.psb.ugent.be

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[Confocal] anti-bleaching mounting medium
by Derui Liu 07 Nov '16

07 Nov '16

Hi all, See this product, may useful to some of you. CitiFluor™ AF1, Mounting Mediumhttp://scienceservices.de/en/citifluortm-af1.htmlhttp://scienceservices.de/en/citifluortm-af1.html Derui Liu - Postdoctoral fellow BrassinosteroidsVIB Department of Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 36 www.psb.ugent.be

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[Confocal] Spinning disc free, feel free to use it. If not I will turn it off in 20min
by Roman HUDECEK 03 Nov '16

03 Nov '16

Roman HUDECEK - Predoctoral fellow Programmed Cell Death VIB Department of Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 16 www.psb.ugent.be

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