February 2017 - confocal@psb.ugent.be

[Confocal] please move data from computer zeiss 710 and exciter to network drives before 16/03 !!
by Evelien Mylle 09 Mar '17

09 Mar '17

Dear confocal users, Could you check whether you still have data on de PC of both Zeiss 710 and Exciter locally and move this to the network programs? There is a lot of data there to find. Please do this before 16/03! After this day, I will delete all data. This action is required for a proper working of the software. Thanks in advance, Sincere greetings, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 www.psb.ugent.be

1 1

[Confocal] evaluation of PC clean up Zeiss 710 (Exciter): no software errors anymore? Please inform me
by Evelien Mylle 27 Feb '17

27 Feb '17

Dear confocal users, The technician from Zeiss came for a check up of the Zeiss 710, as we recently had a serious problem with the software. Some actions has been done and we would like to evaluate this. Could you please report crashes or errors to Daniel or me? If you have other issues to be solved, please communicate, as a maintenance will be done, depending on the system, end of March/ April. This is a good moment to adress them to the company. Many thanks in advance, Don't hesitate to contact Daniel or me in case of problems. Greetings, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 www.psb.ugent.be

1 0

[Confocal] adaptation of switching on Zeiss 710 and Zeiss Exciter to minimize the 'RTC reset intervention' + inform me when you have the error again
by Evelien Mylle 27 Feb '17

27 Feb '17

Dear confocal users, As sometimes there is a problem for starting up the software, mentioning (switch RTC reset button), an adaptation of the switching on procedure is recommanded. These is the case for both Zeiss 710 and Zeiss Exciter. The main principle is that there needs to be some time in between switching the PC on and the components. This allows the Real time controller to get connection . This results in: Zeiss 710: 1. Main switch 2. Systems/PC Wait till computer is on! (f.ex map drives first, but do NOT switch software on) 3. Components Rest can be done as usual Zeiss Exciter: 1. PC components Wait till computer is on! (f.ex map drives first, but do NOT switch software on) 2. System Components Rest can be done as usual I also put a label on the components button mentioning 'wait till PC is on'. Could you please follow this new switching on procedure. Is it also possible to let me know if you would have the error again? This in order to get an idea if the problem is solved. Thanks in advance, Sincere greetings, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 www.psb.ugent.be

1 0

[Confocal] Spinning Disc data folder disapeared
by Derui Liu 26 Feb '17

26 Feb '17

Hey all, Urgently! Does anyone know what happened to the Spinning Disc folder? I could not find it under L:\images\images_cameras anymore, but yesterday when I finished my experiment(around 14;30), it was there. Derui Liu - Postdoctoral fellow BrassinosteroidsVIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 36 www.psb.ugent.be

1 1

[Confocal] Free 710
by Marta Cubria Radio 23 Feb '17

23 Feb '17

Free 710 Feel free to use it! Marta Cubria Radio - Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 14 www.psb.ugent.be

1 0

[Confocal] confocal Zeiss 710 free until 5pm - feel free to use
by Barbara Möller 22 Feb '17

22 Feb '17

Barbara Möller - Postdoctoral fellow Root Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 38 46 www.psb.ugent.be

2 1

[Confocal] Zeiss 710 will be free this afternoon from 1.30pm till 4pm
by Tom Van Hautegem 21 Feb '17

21 Feb '17

Enjoy! Cheers, Tom Tom Van Hautegem - Postdoctoral fellow Systems Biology Of Yield VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 35 29 www.psb.ugent.be

1 0

[Confocal] Zeiss LSM5 Exiter confocal free for the rest of the day
by Joanna Boruc 20 Feb '17

20 Feb '17

Feel free to use it! Joanna Boruc - Postdoctoral fellow Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 www.psb.ugent.be

1 0

[Confocal] Olympus confocal reservation for masim from 11 till 16.00 is cancelled.
by Marta Cubria Radio 20 Feb '17

20 Feb '17

Olympus confocal reservation for masim from 11 till 16.00 is cancelled. Marta Cubria Radio - Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 14 www.psb.ugent.be

2 1

[Confocal] Zeiss 710 will be free from 10am till noon. Feel free to use it!
by Tom Van Hautegem 17 Feb '17

17 Feb '17

Enjoy! Tom Tom Van Hautegem - Postdoctoral fellow Systems Biology Of Yield VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 35 29 www.psb.ugent.be

2 1