September 2017 - confocal@psb.ugent.be

[Confocal] Leica SP2: 515 laser sometimes unstable, adapt % and inform me
by Evelien Mylle 27 Sep '17

27 Sep '17

Dear Leica SP2 users, The 515 laser seems to be unstable: sometimes there is no output at all, whereas this is the case for all other lasers. When this happens, could you move the slider for the % of the 515 laser (in the software) a few times from 0 to 100%. Check if this helps. Please inform me whenever you have this issue. Thanks in advance, Gr, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] rear camera of spinning disc not working
by Evelien Mylle 22 Sep '17

22 Sep '17

Dear spinning disc users, The rear camera (the most used) of the Spinning disc is not working and as consequence also the dual camera mode is not possible. The top camera is still funtional, so an option could be to use this one. The limitation is that only light above 509 nm is send to this camera. (most of the blue fluorophores will not be possible to image) The predefined settings for the top camera are for the moment quit limited (GFP), but more could be setup if desired. If you would encounter problems for the setup, don't hesitate to contact me. Best regards, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] LSM 5 exciter free until 2
by Ambra DE SIMONE 20 Sep '17

20 Sep '17

Feel free to take it Ambra De Simone - Postdoctoral fellow Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 16 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] 710 free Friday 14:00 to 17:00
by Alvaro Fernandez 19 Sep '17

19 Sep '17

Feel free to take it. Alvaro Alvaro Daniel Fernandez Fernandez - Predoctoral fellow Oxidative Stress Signalling VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 22 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] LSM710 free until 11
by Ambra DE SIMONE 18 Sep '17

18 Sep '17

On behalf of Roman Hudecek Ambra De Simone - Postdoctoral fellow Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 16 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Cancelled 710 from 14h to 18h
by Marta Cubria Radio 06 Sep '17

06 Sep '17

Cancelled 710 from 14h to 18h Fell free to use it! Marta Cubria Radio - Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 14 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Spinning disc free from 15:00 onwards
by Joanna Winkler 05 Sep '17

05 Sep '17

Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] LSM710 free this morning until 11 am
by Marlies Huysmans 05 Sep '17

05 Sep '17

Feel free to use! Marlies Huysmans - Predoctoral fellow Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 12 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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