March 2018 - confocal@psb.ugent.be - maillist.psb.ugent.be

[Confocal] Leica SP8: bidirectional scanning requires phase correction
by Evelien Mylle 06 Apr '18

06 Apr '18

Dear Leica SP8 users, Since the upgrade of the software you need to adapt the phaseX correction when doing the bidirectional scanning (see attach how to do). Before the upgrade this was (mostly) never needed, but now this is the case. Without adaptation you get a very strange image (there is a shift from the startposition for each line compared to the previous line, it seems a bit like a splitted image). (when you see it you will notice that it is not good!) There is no fixed value to where you need to change, but probably it is in the range of 20. Look at the image till it looks nice. I will contact the company to ask if we can go back to the situation where no adaptation is required. This information is also available on following folder: [ file://psb.ugent.be/shares/research/images/images_cameras/confocal/Problems_Leica%20SP8/MAIL%20EVMYL | \\psb.ugent.be\shares\research\images\images_cameras\confocal\Problems_Leica SP8\MAIL EVMYL ] In case of problems, don't hesitate to contact Daniel or me. Gr, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] for coming two weeks on 05/04 and 06/04 at work
by Evelien Mylle 30 Mar '18

30 Mar '18

Dear all, Just to inform you that I will only be in the lab on 05/04 and 06/04 for the coming two weeks. Hopefully not, but in case if problems, I will try to solve them on these 2 days. For the week after, you could contact Daniel in case of problems. Greetings, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Leica SP8: map network drive is working again
by Evelien Mylle 28 Mar '18

28 Mar '18

Enjoy! Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Leica SP8 cancelled today from 15:00-17:30
by Joanna Winkler 27 Mar '18

27 Mar '18

Feel free to take it Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] cancelled reservation for spinning disc from 13:00 today
by Derui Liu 26 Mar '18

26 Mar '18

feel free to take it. Derui Liu - Postdoctoral fellow Brassinosteroids VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 36 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Feel free to use the confocal 710 till 14h
by Marta Cubria Radio 26 Mar '18

26 Mar '18

Feel free to use the confocal 710 till 14h, I will start using it from then on. Marta Cubria Radio - Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 14 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] SP8X free on Monday from 10-13h
by Rafael Buono 25 Mar '18

25 Mar '18

Take it if you want it. Best, Rafael Rafael Andrade Buono - Postdoctoral fellow Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 16 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Spinning disk free until 17:30
by Joanna Winkler 23 Mar '18

23 Mar '18

Feel free to take it Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Leica Sp2: 514 laser is unstable, sometimes it works, sometimes not. Other lasers are fine.
by Evelien Mylle 22 Mar '18

22 Mar '18

Dear confocal lasers, The 514 laser from the Leica SP2 gives unstable output. Sometimes working nicely, other times not. The other lasers are working nicely. Depending on your dye or fluorophore it could be possible to excite your sample by using another laserline. Leica SP2 has also a 496 laser which is the closest to the 514 laser. The company is informed and I will keep you updated. Best regards, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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[Confocal] Leica SP8: panel showing laserlines & selecting laserlines is changed
by Evelien Mylle 22 Mar '18

22 Mar '18

Dear Leica SP8 users, The layout where you select your laserlines is changed dued to upgrade of the LasX software. The way to use is still the same, but it just looks a bit different. In attach more info. Enjoy, Gr, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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