May 2018 - confocal@psb.ugent.be - maillist.psb.ugent.be

Re: [Confocal] Zeiss 710: What to do in case if argon laser doesn't work
by Evelien Mylle 17 May '18

17 May '18

> Dear all, > To follow up the problem we had with the argon laser we need to find out what > exactly is not working from the argon laser. > Therefore it is important to have the following information (if the argon laser > is not working): > - which colour do you see at the box of the argon laser: purple with a bit of > orange or only orange? > - which LEDs are on at the front panel > See attach for more info. > Please contact Daniel or me in case of problems. If we are not around, you could > take a picture of the box of the argon laser (to see the colour) and of the > front panel. > Gr, > Evelien >> From: "Evelien Mylle" <evmyl(a)psb.ugent.be> >> To: Confocal(a)psb.ugent.be >> Cc: "Daniel Van Damme" <dadam(a)psb.vib-ugent.be> >> Sent: Wednesday, May 16, 2018 10:05:36 AM >> Subject: For the moment argon laser is working: Re:Zeiss 710: argon laser not >> working >> Hello, >> Today the argon laser is working again. Perhaps there is an unstable component >> or ...? >> We have to follow this up. So please keep Daniel and me informed in case if you >> encounter again a problem. >> Thanks, >> Evelien >>> From: "Evelien Mylle" <evmyl(a)psb.ugent.be> >>> To: Confocal(a)psb.ugent.be >>> Cc: "Daniel Van Damme" <dadam(a)psb.vib-ugent.be> >>> Sent: Tuesday, May 15, 2018 6:12:02 PM >>> Subject: Zeiss 710: argon laser not working >>> Dear confocal users, >>> The argon laser of the Zeiss 710 was not working this afternoon. >>> No idea about the reason, so don't hesitate to try again. You never know... >>> Greetings, >>> Evelien >>> Evelien Mylle - Lab Manager / Technician >>> Advanced Live Cell Imaging >>> VIB-UGent Center for Plant Systems Biology >>> Ghent University >>> Technologiepark 927 - 9052 Ghent - Belgium >>> Tel. +32(0)9 331 39 34 >>> [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 0

[Confocal] SP8 free till 11h
by Amna Mhamdi 17 May '18

17 May '18

Hi, feel free to use the SP8, I only need it at 11h. Best regards, Amna Amna M'Hamdi - Postdoctoral fellow Oxidative Stress Signalling VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 25 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

2 1

[Confocal] Zeiss 710: argon laser not working
by Evelien Mylle 16 May '18

16 May '18

Dear confocal users, The argon laser of the Zeiss 710 was not working this afternoon. No idea about the reason, so don't hesitate to try again. You never know... Greetings, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 1

[Confocal] SD: slow imaging
by Evelien Mylle 14 May '18

14 May '18

Dear spinning disc users, We have a problem with our spinning disc as the imaging takes much longer than should be the case. F.ex. One channel and one slice makes the imaging around 500 msec longer than what should be the case (here is no need for changing filter or beamsplitter, so only the exposure time determines the imaging time). These observations were done with the rear camera. It's not checked whether also the top camera does this. Please keep this in mind when doing your experiments. In principle everything can still be done, but much slower. Depending on your experiment this can be doable or problematic. Company is informed and I will keep you informed. Greetings, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 1

[Confocal] 710 FREE today from 14.30 to 17.30
by Marta Cubria Radio 14 May '18

14 May '18

Feel free to use it! Cheers, Marta Cubria Radio - Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 14 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

2 1

[Confocal] “Luxaflex” usb stick found at SP8
by Jos Wendrich 08 May '18

08 May '18

Jos Wendrich, PhD Post-Doc PSB, VIB-UGent Vascular Development Technologiepark Zwijnaarde 927 9052 Ghent, Belgium Email: jos.wendrich(a)psb.ugent.be Phone: +32 9 331 39 43 http://www.psb.ugent.be/vascular-development Google Scholar: https://scholar.google.nl/citations?user=Cqs0p58AAAAJ&hl=nl Research Gate: https://www.researchgate.net/profile/Jos_Wendrich LinkedIn: https://nl.linkedin.com/in/jos-wendrich-29642416

1 0

[Confocal] Leica SP8 free today from 9:00 - 12:30
by Astrid Gadeyne 08 May '18

08 May '18

Astrid Gadeyne - Postdoctoral fellow Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 38 74 www.psb.ugent.be

2 1

[Confocal] spinning disk free
by Joanna Winkler 07 May '18

07 May '18

>From now on, Greetigns, Joanna Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 0

[Confocal] Zeiss 710: argon laser doesn't not always switch on - if on, do NOT switch off!
by Evelien Mylle 04 May '18

04 May '18

Dear confocal users, The argon laser of Zeiss 710 has problems to switch on. As the laser has almost 7000 working hours, I think this is the beginning of the end for this laser. Monday there was a similar problem, but on Tuesday, when the technician of Zeiss was here, the switching on was fine. Today again there was a problem with switching on. The first sign of degradation is often problem with switching on. I can only advice to try several times to get it on, either by restarting up completely. Or, and I think this could be more more helpfull, by switching the system on, leave it on and hoping that the laser will go on after a while. If someone succeeds in getting the laser on, please DO NOT SWITCH OFF!!! (even for the weekend!) It can be left on till replacement will be done. The company will be contacted for the installation of a new laser. Greetings, Evelien Evelien Mylle - Lab Manager / Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 1

[Confocal] Looking for people with experience with long timelaps using Leica SP8
by Anaxi Houbaert 02 May '18

02 May '18

Hello dear SP8 confocal users I'm looking for someone who tried long (>12h) timelaps on the SP8. I would like to image cotyledon development using 1.5-2days old seedlings. I would like to hear feedback on the protocol and settings of the microscope. Many thanks in advance! Anaxi Anaxi Houbaert - Predoctoral fellow Brassinosteroids VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 36 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 0