July 2018 - confocal@psb.ugent.be - maillist.psb.ugent.be

[Confocal] Spinning disc: Top camera not working
by Evelien Mylle 29 May '19

29 May '19

Dear spinning disc, The Top camera is not working. Please keep this in mind for your experiments. You still can use the rear camera (what most people use I think), but top and dual camera mode is not working. As usual I will keep you informed and hope to have this solved rather fast. Greetings, Evelien Evelien Mylle - Lab Manager / Microscopy Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

2 4

[Confocal] 710 free today and tomorrow morning due to failed experiment
by Lucas ALVES NEUBUS CLAUS 30 Jul '18

30 Jul '18

Dear All, Due to failed experiment I won't be using the 710 today and tomorrow morning (9:00-12:30). Feel free to take it. Best, Lucas Lucas Alves Neubus Claus - Predoctoral fellow Brassinosteriods VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 36 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 0

[Confocal] Leica SP8 free from now on, I will switch off within 15 min if nobody uses it
by Evelien Mylle 25 Jul '18

25 Jul '18

Enjoy Evelien Mylle - Lab Manager / Microscopy Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

2 1

[Confocal] Leica SP8: gating not always working on HyD1
by Evelien Mylle 24 Jul '18

24 Jul '18

Dear all, In the last months there has been 3 times the issue of a non-working gating on HyD1. Keep this in mind when you use the gating. If no change is observed when applying gating, make sure to find out if gating is working (f.ex. by checking a leaf and try to exclude the chlorophyll with gating). Restarting the system solved the problem. Could you also register the problem: -Inform Daniel or me. -Picture with and without gating and save it in a new folder with date and information on \\psb.ugent.be\shares\research\images\images_cameras\confocal\Problems_Leica SP8. -Also create an error report by clicking on Help (in the LAS X software, choose 'create error report'. Save it into the same folder. Thanks in advance, Best regards, Evelien Evelien Mylle - Lab Manager / Microscopy Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 0

[Confocal] Exciter free from 14:00 today and 9:00-14:00 tomorrow
by ke xu 23 Jul '18

23 Jul '18

Samples are contaminated. Cancelled my reservation. Free to take. Ke Xu - Predoctoral fellow Root Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)93313999 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

2 1

[Confocal] Zeiss 710: air table not optimal
by Evelien Mylle 13 Jul '18

13 Jul '18

Dear confocal users, One part of the air-microscopy table is broken (t he left back corner). A wooden block is temporary replacing the broken air mount. Imaging can be performed as usual. But it could be you will see more effect of vibration during imaging. (As the air table is meant to avoid vibration to your sample). For this avoid touching or pushing on the table during imaging. Gr, Evelien Evelien Mylle - Lab Manager / Microscopy Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 0

[Confocal] Zeiss 710: slightly blurry images
by Evelien Mylle 13 Jul '18

13 Jul '18

Dear confocal users, We noticed that the Zeiss 710 gives some 'unsharp' images. In attach example of image taken with Zeiss 710 and Exciter. Please judge yourself if you still can use it for your experiments. I will keep you informed. Greetings, Evelien Evelien Mylle - Lab Manager / Microscopy Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 1

[Confocal] Zeiss 710: detector (channel) 2 NOT working
by Evelien Mylle 13 Jul '18

13 Jul '18

Dear confocal users, Channel or detector 2 from Zeiss 710 is not working. Depending on the number of fluorophores, you can continu imaging but with a small adaptation of the Light path: For imaging up to 2 colours just use the other 2 channels (Ch1 and Ch3). For imaging 3 colours FRAME sequential scanning will be required ( one of the channels will capture 2 colours, or you can even put all colours on one channel). LINE sequential imaging or simultanous imaging will not be possibe. Also note that in case of Smart setup or reuse, you will have to adapt the Light path. Don't hesitate to contact Daniel or me if you would have problems for setting up. Gr, Evelien Evelien Mylle - Lab Manager / Microscopy Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 2

[Confocal] Leica SP8 free until 4pm
by Joanna Winkler 09 Jul '18

09 Jul '18

feel free to take it Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 0

[Confocal] LSM5 confocal
by Anchal Bisht 05 Jul '18

05 Jul '18

LSM5 is free in the afternoon. Feel free to use it ! Anchal Bisht - Predoctoral fellow Cell Cycle VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 64 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

1 0