November 2019 - confocal@psb.ugent.be

[Confocal] SD: dichroic exchanged for simultaneously imaging CFP/YFP, instead of GFP/RFP
by Evelien Mylle 23 Feb '21

23 Feb '21

Dear all, The 509 beamsplitter is now installed allowing to simultaneously image CFP/YFP. If you would like to simultaneously image GFP/RFP the beamsplitter needs to be changed to the 580 beamsplitter. This is physically taking out the filter and put the other one in, something that needs to be done by Daniel or me. Keep in mind that before using both cameras you ALWAYS need to calibrate first. This can be done through a userfriendly wizard 'Calibrate Multicamera' (under Video). If not clear, don't hesitate to ask for help. Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SD: issue with rear camera, cameras exchanged, please contact me when you will use it
by Evelien Mylle 13 Mar '20

13 Mar '20

Dear all, The cameras of the spinning disc are exchanged as the rear camera (the mostly used one) gives a very pixeled image. The top camera is now on the place of the rear camera, but settings are for the moment still as top camera. With some adaptations you can use it as normal. Please contact me if you cant to use it, so I can help you with this. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8: hopefully also solved: error : Firmware event code: timeout image transfer
by Evelien Mylle 17 Dec '19

17 Dec '19

Dear all, If the following error (firmware event code, see picture below) would still appear, please let me know!! In principle, this shouldn't be the case anymore. This error was related to the problem with the part causing the problem for the brightfield light. As this part is completely new, we shouldn't have it anymore. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica sp8 cancelled on 6th Dec
by Joanna Winkler 29 Nov '19

29 Nov '19

Greetings, Joanna Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 73 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 is free 2/12 morning (9:00-13:00)
by Lucas ALVES NEUBUS CLAUS 29 Nov '19

29 Nov '19

Feel free to take it. Lucas Alves Neubus Claus - Predoctoral fellow Brassinosteriods VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 36 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Free spot 2/12 From 15:00 - 19:00
by Roman HUDECEK 29 Nov '19

29 Nov '19

Feel free to take it Roman Hudecek - Predoctoral fellow Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 15 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP2: transmission light source brakes rather fast
by Evelien Mylle 27 Nov '19

27 Nov '19

Dear SP2 users, The transmission light of the SP2 seems to break rather fast (already 2 lamps replaced in the last month, which is exceptional). It can be solved by replacing the lamp. Please come to Daniel or me as we can easily exchange it. If we are not around, you can also ask Kristof (techhelp). A working transmission light gives a visible light at the left side of the microscope (see indicated black box at picture below). You only need this for visualizing your sample through the microscope (NOT for confocal imaging). Thanks in advance, Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] sp8 reservation cancelled
by Eliana Mor 22 Nov '19

22 Nov '19

26/11 h15-17 28/11 h14-17 Feel free to use it Eliana Mor - Predoctoral fellow Vascular Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 44 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8X is free (12:30-43:00)
by Jelle Van Leene 22 Nov '19

22 Nov '19

Jelle Van Leene - Staff scientist [ https://www.psb.ugent.be/functional-interactomics | Functional Interactomics ] [ https://www.psb.ugent.be/functional-interactomics ] VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 38 75 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8X is free (12:30-13:00)
by Jelle Van Leene 22 Nov '19

22 Nov '19

Jelle Van Leene - Staff scientist [ https://www.psb.ugent.be/functional-interactomics | Functional Interactomics ] [ https://www.psb.ugent.be/functional-interactomics ] VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 38 75 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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