November 2019 - confocal@psb.ugent.be

[Confocal] Zeiss Exciter: CH1 not working, FRAME sequential imaging can be used
by Evelien Mylle 08 Nov '19

08 Nov '19

Dear all, The Ch1 from the Zeiss Exciter is not working anymore. This influences mainly the 2-colour imaging , and mainly your red dye or fluorophore. One colour imaging can be done by sending everything to the first detector (Ch2), meaning that reuse of the settings of a previous image could need some adaptation. Two colour imaging could be done by FRAME sequential imaging : both your dyes will be captured at the same channel, but one after the other. Keep in mind that there is more time-delay between imaging your 2 colours compared to the simultanous or line sequential mode, which is normally used. During live mode I would recommand to only use one channel at a time as it takes more time to see the effect in the second channel. This can be easily done in the software by activating-deactivating a channel (also explained in the ppt in attach). More information in attach and also in the help folder, including a picture with settings allowing to reload the settings for GFP - PI FRAME sequential imaging: S:\research\images\images_cameras\confocal\Help Zeiss Exciter\20191108_Frame sequential scanning GFP PI\ GFP and PI_Frame sequential.lsm Don't hesitate to contact Daniel or me if you would have problems with the lightpath setup. I will keep you update about the repair of this channel. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica sp8 free until 12 today
by Joanna Winkler 07 Nov '19

07 Nov '19

Greetings, Joanna Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 73 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leice SP8 Free today overnight and tomorrow overnight
by Balkan Canher 07 Nov '19

07 Nov '19

Dear All I won't be able to use my reservations with Leice SP8 Free today overnight and tomorrow overnight. Feel Free to take it. Kind Regards, Balkan Canher - Predoctoral fellow Cell Cycle VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8 free from 14 - 18:00 this afternoon
by Darja STOJANOVOVA 04 Nov '19

04 Nov '19

Please feel free to use Darja Stojanovova - Predoctoral fellow Cell Cycle VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 36 93 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] LSM710 free today 12-13
by Matous GLANC 04 Nov '19

04 Nov '19

Matous Glanc - Postdoctoral fellow Vascular Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 45 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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