May 2019 - confocal@psb.ugent.be - maillist.psb.ugent.be

[Confocal] please register overnight sessions as real overnight!
by Evelien Mylle 21 Aug '19

21 Aug '19

Dear confocal users, Could you register overnight sessions by booking the real time you will use it ? (so NOT by reserving till 20:00 and typing ON!) Reason is that the booking is the only calculation we have to convince the management that the machines we have are adequately used. It is therefore in everyone's interest to make sure that these numbers are as close to reality. I do assume nobody will come to shut down the lasers at 2am... If you don't have enough credits, you can ask your groupleader to make some reservations. (every groupleader gets extra credits to cover this). Thanks in advance, Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica sp8 free from 1.00pm-3.00pm
by Joanna Winkler 31 May '19

31 May '19

Greetings, Joanna Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 73 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Spinning disc: Top camera not working
by Evelien Mylle 29 May '19

29 May '19

Dear spinning disc, The Top camera is not working. Please keep this in mind for your experiments. You still can use the rear camera (what most people use I think), but top and dual camera mode is not working. As usual I will keep you informed and hope to have this solved rather fast. Greetings, Evelien Evelien Mylle - Lab Manager / Microscopy Technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark 927 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]

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Re: [Confocal] Confocal ZEISS 710 canceled, fell free to take it
by Baojun Yang 28 May '19

28 May '19

I will take it, Thanks! Baojun > From: "Xingliang Duan" <xingliang.duan(a)psb.vib-ugent.be> > To: "wetlab" <wetlab(a)psb.vib-ugent.be> > Sent: Tuesday, May 28, 2019 12:17:25 PM > Subject: Confocal ZEISS 710 canceled, fell free to take it > Xingliang duan - Predoctoral fellow > Oxidative Stress Signaling > VIB-UGent Center for Plant Systems Biology > Ghent University > Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium > Tel. +32(0)9 331 39 22 > [ http://www.psb.ugent.be/ | www.psb.ugent.be ] > Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] LSM 710 is free today and tomorrow - from 12h to14h
by Ana Rita Leal 27 May '19

27 May '19

I will delete my bookings, Enjoy it! Rita Ana Rita Mendes Leal - Predoctoral fellow Root Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 05 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 X free this afternoon
by Qiaozhi Yu 24 May '19

24 May '19

Feel free to take it. Qiaozhi Yu - Predoctoral fellow Root Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 38 46 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 is Free
by Norbert BOLLIER 20 May '19

20 May '19

Hi all, The SP8X is free for use from now. If I get no answer within 30min, I will turn it off. Best, Norbert Norbert BOLLIER - Postdoctoral fellow Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 12 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8X free
by Rafael Buono 17 May '19

17 May '19

SP8X is free for use. If I get no answer within 30min, I will turn it off. best, Rafael Rafael Andrade Buono - Postdoctoral fellow Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 16 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Zeiss710: 'jumps' in line formation of image
by Evelien Mylle 16 May '19

16 May '19

Dear Zeiss 710 confocal users, There is an issue with the scanning on the Zeiss 710, causing some 'jumps' in the image formation. Please take a look at the pictures. The jumps are indicated with an arrow. I assume this is also related to the noise we recently hear during the scanning. A temporary solution (or perhaps partially) would be to scan at rotation 90° in stead of rotation 0, as this makes more use of the Y scanhead in stead of the X scanhead, which is probably a bit overworked. Also the noise in this setup is less. In the test sample I observed jumps when scanning with rotation 0° , but not with rotation 90°. (but could be also dued to sample as in this direction jumps are more difficult to see) The company is contacted and I will update you. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] ZEISS 710 free from now till 11.00h
by Sarah Langendries 16 May '19

16 May '19

Sarah Langendries - Predoctoral fellow Rhizosphere VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 08 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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