June 2019 - confocal@psb.ugent.be - maillist.psb.ugent.be

[Confocal] SD: dichroic exchanged for simultaneously imaging CFP/YFP, instead of GFP/RFP
by Evelien Mylle 23 Feb '21

23 Feb '21

Dear all, The 509 beamsplitter is now installed allowing to simultaneously image CFP/YFP. If you would like to simultaneously image GFP/RFP the beamsplitter needs to be changed to the 580 beamsplitter. This is physically taking out the filter and put the other one in, something that needs to be done by Daniel or me. Keep in mind that before using both cameras you ALWAYS need to calibrate first. This can be done through a userfriendly wizard 'Calibrate Multicamera' (under Video). If not clear, don't hesitate to ask for help. Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] please register overnight sessions as real overnight!
by Evelien Mylle 21 Aug '19

21 Aug '19

Dear confocal users, Could you register overnight sessions by booking the real time you will use it ? (so NOT by reserving till 20:00 and typing ON!) Reason is that the booking is the only calculation we have to convince the management that the machines we have are adequately used. It is therefore in everyone's interest to make sure that these numbers are as close to reality. I do assume nobody will come to shut down the lasers at 2am... If you don't have enough credits, you can ask your groupleader to make some reservations. (every groupleader gets extra credits to cover this). Thanks in advance, Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SD: if laser(s) would not work, make movie of the lights of the laserbox
by Evelien Mylle 18 Jul '19

18 Jul '19

Dear spinning disc users, Lately we observed 2 to 3 times that one or all the lasers were not working. Restarting the system solved the problem. In order to solve this issue, I'm in contact with the company and need the following information: - What are the lights from the laserbox doing on the moment that the laser(s) is/are not working? The best would be to take a movie/picture of the laserbox: one with the system on but no imaging, and one were you are imaging. Thanks in advance! Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8X free Wednesday and Thursday times
by Rafael Buono 25 Jun '19

25 Jun '19

Hi! Experiment did not work. Times for Wednesday and Thursday afternoon just opened up for SP8X. best, Rafael Rafael Andrade Buono - Postdoctoral fellow Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 16 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] leica sp8
by Darja STOJANOVOVA 25 Jun '19

25 Jun '19

Hi, The Leica SP8 is now free on Thursday 10 - 13:30. Best Darja Darja Stojanovova - Predoctoral fellow Cell Cycle VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 36 93 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] sp2 free
by Eliana Mor 24 Jun '19

24 Jun '19

tomorrow 25th from 13-15.30 Eliana Mor - Predoctoral fellow Vascular Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 44 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] LEICA SP8 free until 13:00
by Barbara Möller 24 Jun '19

24 Jun '19

Barbara Möller - Postdoctoral fellow Root Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 38 46 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 from 9 am to 13 pm next Wednesday (26/06) cancelled
by Qing Lu 23 Jun '19

23 Jun '19

Qing Lu - Predoctoral fellow Brassinosteroids VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 36 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Zeiss 710: what to do when lasers are not working
by Evelien Mylle 21 Jun '19

21 Jun '19

Dear Zeiss 710 users, Could you come and get me in case you have non-working lasers, which seemed to be combined with following info message: If I am not around, could you do a Cancheck: (these information is also available in Zeiss 710 problems folder (\\psb.ugent.be\shares\research\images\images_cameras\confocal\Problems_Zeiss710\20190914_Laser off) . Hopefully you can solve the issue by checking that the arm of the confocal is positioned well. Thanks in advance, Best imaging regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 free from 12:00-14:00
by Roman HUDECEK 20 Jun '19

20 Jun '19

Feel free to take it Roman Hudecek - Predoctoral fellow Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 15 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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