July 2019 - confocal@psb.ugent.be - maillist.psb.ugent.be

[Confocal] SD: dichroic exchanged for simultaneously imaging CFP/YFP, instead of GFP/RFP
by Evelien Mylle 23 Feb '21

23 Feb '21

Dear all, The 509 beamsplitter is now installed allowing to simultaneously image CFP/YFP. If you would like to simultaneously image GFP/RFP the beamsplitter needs to be changed to the 580 beamsplitter. This is physically taking out the filter and put the other one in, something that needs to be done by Daniel or me. Keep in mind that before using both cameras you ALWAYS need to calibrate first. This can be done through a userfriendly wizard 'Calibrate Multicamera' (under Video). If not clear, don't hesitate to ask for help. Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] please register overnight sessions as real overnight!
by Evelien Mylle 21 Aug '19

21 Aug '19

Dear confocal users, Could you register overnight sessions by booking the real time you will use it ? (so NOT by reserving till 20:00 and typing ON!) Reason is that the booking is the only calculation we have to convince the management that the machines we have are adequately used. It is therefore in everyone's interest to make sure that these numbers are as close to reality. I do assume nobody will come to shut down the lasers at 2am... If you don't have enough credits, you can ask your groupleader to make some reservations. (every groupleader gets extra credits to cover this). Thanks in advance, Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8 free until 12am on Wednesday
by Joanna Winkler 29 Jul '19

29 Jul '19

Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 73 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Spinning disk free until 3pm
by Joanna Winkler 26 Jul '19

26 Jul '19

Feel free to take it. Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 73 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP2: issue 'can not load personal settings'
by Evelien Mylle 19 Jul '19

19 Jul '19

Dear SP2 users, A recurring problem is 'can not load personal settings'. In attach a description how to solve this. Most probably this problem occurs by not connecting the PC correctly: log in to the PC (NOT PSB) with administrator username. Software should be loaded with personal settings. Please follow the correct procedure. All information is available in the HELP LEIca SP2 folder: \\psb.ugent.be\shares\research\images\images_cameras\confocal\Help Leica SP2 Thanks in advance, Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8X is free
by Helena Arents 19 Jul '19

19 Jul '19

Hey everyone, The SP8 is free and I cancelled my afternoon reservation. Feel free to use it. Greetings, Helena Helena E. Arents - Predoctoral fellow Vascular Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 45 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SD: if laser(s) would not work, make movie of the lights of the laserbox
by Evelien Mylle 18 Jul '19

18 Jul '19

Dear spinning disc users, Lately we observed 2 to 3 times that one or all the lasers were not working. Restarting the system solved the problem. In order to solve this issue, I'm in contact with the company and need the following information: - What are the lights from the laserbox doing on the moment that the laser(s) is/are not working? The best would be to take a movie/picture of the laserbox: one with the system on but no imaging, and one were you are imaging. Thanks in advance! Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Olympus confocal cancelled for Monday and Tuesday 15 and 16/07
by Evelien Mylle 10 Jul '19

10 Jul '19

Feel free to use! Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP2 free tomorrow 13:30-16:30
by Matous GLANC 09 Jul '19

09 Jul '19

I will use SP8 on Thursday instead Matous Glanc - Postdoctoral fellow Vascular Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 45 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 from 13:00 to 17:00 this Thursday (11/07) cancelled
by Qing Lu 09 Jul '19

09 Jul '19

Qing Lu - Predoctoral fellow Brassinosteroids VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 36 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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