September 2019 - confocal@psb.ugent.be

[Confocal] SD: dichroic exchanged for simultaneously imaging CFP/YFP, instead of GFP/RFP
by Evelien Mylle 23 Feb '21

23 Feb '21

Dear all, The 509 beamsplitter is now installed allowing to simultaneously image CFP/YFP. If you would like to simultaneously image GFP/RFP the beamsplitter needs to be changed to the 580 beamsplitter. This is physically taking out the filter and put the other one in, something that needs to be done by Daniel or me. Keep in mind that before using both cameras you ALWAYS need to calibrate first. This can be done through a userfriendly wizard 'Calibrate Multicamera' (under Video). If not clear, don't hesitate to ask for help. Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SD: issue with rear camera, cameras exchanged, please contact me when you will use it
by Evelien Mylle 13 Mar '20

13 Mar '20

Dear all, The cameras of the spinning disc are exchanged as the rear camera (the mostly used one) gives a very pixeled image. The top camera is now on the place of the rear camera, but settings are for the moment still as top camera. With some adaptations you can use it as normal. Please contact me if you cant to use it, so I can help you with this. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8: sometimes problem with microscope settings
by Evelien Mylle 20 Nov '19

20 Nov '19

Dear all, The Leica SP8 gives sometimes problems when looking through the microscope to your sample: there is less light and you don't get your sample nicely in focus. It can be solved by clicking (several times) on live view (in the LAS X software), and/or by switching the lens, and/or switching between DIC and POL light (microscope setting). Closing and restarting the PC sometimes helps, but not always, and it is very time consuming. So, I would go for the above options. I assume that there is some kind of filter that gets blocked. Keep this in mind when doing your imaging especially if you are doing treatments, as it could take a bit more time to set up your imaging. I will keep you updated. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP2: Argon laser not working anymore
by Evelien Mylle 01 Oct '19

01 Oct '19

Dear SP2 users, The argon laser (with lines 458,476,488,496,514) is not working anymore. The other lines (561, 594 and 633) are fine. Please keep this in mind for your experiments. The PI imaging, which is often done on this confocal, can still be done using the 561 to excite. GFP-imaging is not possible anymore. I will inform you when the new laser is installed. Best greetings, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 reservation MONDAY from 8:00-12:30 deleted
by Helena Arents 30 Sep '19

30 Sep '19

Dear wetlab Cancelled my monday reservation, feel free to take it. Helena E. Arents - Predoctoral fellow Vascular Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 45 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Olympus confocal session cancelled for this afternoon, feel free to use
by Evelien Mylle 24 Sep '19

24 Sep '19

Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Zeiss LSM 710 confocal free til 12h
by Sarah Langendries 12 Sep '19

12 Sep '19

Sarah Langendries - Predoctoral fellow Rhizosphere VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 08 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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Re: [Confocal] LSM710 free now until 11:00
by Fei XIE 11 Sep '19

11 Sep '19

I will take it! Cheers, fei > From: "Fei XIE" <fei.xie(a)psb.ugent.be> > To: "Matous GLANC" <matous.glanc(a)psb.vib-ugent.be> > Sent: Wednesday, September 11, 2019 10:16:49 AM > Subject: Re: [Confocal] LSM710 free now until 11:00 > I will take it! > Cheers, > fei >> From: "Matous GLANC" <matous.glanc(a)psb.vib-ugent.be> >> To: "confocal" <confocal(a)psb.vib-ugent.be> >> Sent: Wednesday, September 11, 2019 9:21:22 AM >> Subject: [Confocal] LSM710 free now until 11:00 >> Change of plans, sorry for late notice! >> Matous Glanc - Postdoctoral fellow >> Vascular Development >> VIB-UGent Center for Plant Systems Biology >> Ghent University >> Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium >> Tel. +32(0)9 331 39 45 >> [ http://www.psb.ugent.be/ | www.psb.ugent.be ] >> Genome editing, cutting-edge technology for a sustainable agriculture >> _______________________________________________ >> Confocal mailing list >> Confocal(a)psb.ugent.be >> https://maillist.psb.ugent.be/mailman/listinfo/confocal

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[Confocal] LSM710 free now until 11:00
by Matous GLANC 11 Sep '19

11 Sep '19

Change of plans, sorry for late notice! Matous Glanc - Postdoctoral fellow Vascular Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 45 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Zeiss LSM 710 confocal FREE untill 16h
by Sarah Langendries 10 Sep '19

10 Sep '19

Sarah Langendries - Predoctoral fellow Rhizosphere VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 08 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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