October 2020 - confocal@psb.ugent.be

[Confocal] SD: dichroic exchanged for simultaneously imaging CFP/YFP, instead of GFP/RFP
by Evelien Mylle 23 Feb '21

23 Feb '21

Dear all, The 509 beamsplitter is now installed allowing to simultaneously image CFP/YFP. If you would like to simultaneously image GFP/RFP the beamsplitter needs to be changed to the 580 beamsplitter. This is physically taking out the filter and put the other one in, something that needs to be done by Daniel or me. Keep in mind that before using both cameras you ALWAYS need to calibrate first. This can be done through a userfriendly wizard 'Calibrate Multicamera' (under Video). If not clear, don't hesitate to ask for help. Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SD: other joystick, no dual camera, please report problems from coming weeks
by Evelien Mylle 27 Nov '20

27 Nov '20

Dear spinning disc users, Here are some temporary changes to our spinning disc setup: - other joystick - No dual camera setup possible These adjustments are the result of installation of the loan proscan unit. The purpose is to check if the crashes of the volocity software are due to our proscan unit or not. So it is very important to know how the system will behave in the coming period. So PLEASE report and register ALL PROBLEMS ( print screen, session error log, ....)! And inform me about it. As usual you can save this under \psb.ugent.be\shares\research\images_cameras\spinning disc\Problems Spinning disc I will keep you updated about any change. Hoping that the dual camera setup will soon be available. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] olympus confocal: IMPORTANT!! switching off adapted procedure!!!
by Evelien Mylle 25 Nov '20

25 Nov '20

Dear Olympus users, @ Ignachio, can you confirm as you are the last user today. (I could not find you in the lab) Please follow the instructions in picture below or in attach in order to switch ONLY the 559 and fluorescence lamp off, so LEAVING THE ARGON LASER ON!! Reason is the following: The argon laser took this morning more than one hour to get properly working. THis, together with the non -working 515 laser, makes me thinks that the argon laser is at his end. >From experience, not getting the laser on is often one of the first signs. Once it's on, it's all OK. That's why I adjust the switching off procedure by leaving the laser on! It is a bit risky, with a possible lockdown in prospect, but I don't want to take the risk of not being able to activate the laser on Monday. The reservations are full for next week, and possibly these are experiments that will be finalized next week. Do switch off the 559 and the fluorescence lamp! See cadaped switching off procedure below: Gr, Evelien Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] LSM710 free until 11:30
by Anchal Bisht 30 Oct '20

30 Oct '20

Anchal Bisht - Predoctoral fellow Cell Cycle VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 64 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Sp8 free until 6pm
by Joanna Winkler 29 Oct '20

29 Oct '20

Greetings, Joanna Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 73 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8: please ignore important security notice
by Evelien Mylle 27 Oct '20

27 Oct '20

Dear all, You can ignore the following SP8 important security notice message (see below). Upon upgrade/ intervention this will be removed. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 free on thursday from 17:00 till 20:00
by Jonas DE BACKER 27 Oct '20

27 Oct '20

I have to cancel my spot because my agrobacteria didn`t grow, feel free to reserve it Kind regards, Jonas De Backer Jonas De Backer - Predoctoral fellow Oxidative Stress Signaling VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 33 92 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] confocals free
by Matous GLANC 26 Oct '20

26 Oct '20

Olympus today 15:30-19:30 Leica SP8 Friday 30th 10:00-16:00 Enjoy! M Matouš Glanc - Postdoctoral fellow Vascular Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 45 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 cancelled
by Baojun Yang 26 Oct '20

26 Oct '20

SP8: 26th: 20:00-22:00 27th:19:00-22:00 28th:19:00-22:00 Feel free to use! Baojun Yang - Postdoctoral fellow Vascular Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 43 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP2 free this morning
by Jos Wendrich 26 Oct '20

26 Oct '20

I had to cancel my booking for this morning. Feel free to book and use. Jos Jos Wendrich, PhD Post-Doc PSB, VIB-UGent Vascular Development Technologiepark Zwijnaarde 71 9052 Ghent, Belgium Email: jos.wendrich(a)psb.ugent.be Phone: +32 9 331 39 43 http://www.psb.ugent.be/vascular-development Google Scholar: https://scholar.google.nl/citations?user=Cqs0p58AAAAJ&hl=nl LinkedIn: https://linkedin.com/in/jos-wendrich ORCID: https://orcid.org/0000-0002-6198-3763 Research Gate: https://www.researchgate.net/profile/Jos_Wendrich

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