December 2020 - confocal@psb.ugent.be

[Confocal] SD: dichroic exchanged for simultaneously imaging CFP/YFP, instead of GFP/RFP
by Evelien Mylle 23 Feb '21

23 Feb '21

Dear all, The 509 beamsplitter is now installed allowing to simultaneously image CFP/YFP. If you would like to simultaneously image GFP/RFP the beamsplitter needs to be changed to the 580 beamsplitter. This is physically taking out the filter and put the other one in, something that needs to be done by Daniel or me. Keep in mind that before using both cameras you ALWAYS need to calibrate first. This can be done through a userfriendly wizard 'Calibrate Multicamera' (under Video). If not clear, don't hesitate to ask for help. Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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Re: [Confocal] SP8 gating
by evmyl＠psb.vib-ugent.be 31 Dec '20

31 Dec '20

Hey,Please verify that the proper laser is selected for that detector (where you have the gating on,off and the borders of the gating you also can choose a laser.If still not ok, restart the software. If problem persists, than I will take a look next week.Please keep me informed. GrEvelien -------- Oorspronkelijk bericht --------Van: Alvaro Fernandez <Alvaro.Fernandez(a)psb.vib-ugent.be> Datum: 31/12/20 12:45 (GMT+01:00) Aan: confocal <confocal(a)psb.vib-ugent.be> Onderwerp: [Confocal] SP8 gating Dear users,Today in the SP8 there was a problem with the gating, there is no difference between gating and no gating so I think the configuration has changed.If there is anyone knowing how to solve it please let us know.Otherwise please consider this when imaging.Cheers,Alvaro Alvaro Daniel Fernandez Fernandez - Predoctoral fellow Oxidative Stress Signalling VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 22 www.psb.ugent.be Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 gating
by Alvaro Fernandez 31 Dec '20

31 Dec '20

Dear users, Today in the SP8 there was a problem with the gating, there is no difference between gating and no gating so I think the configuration has changed. If there is anyone knowing how to solve it please let us know. Otherwise please consider this when imaging. Cheers, Alvaro Alvaro Daniel Fernandez Fernandez - Predoctoral fellow Oxidative Stress Signalling VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 22 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 free till 17h00
by Boyu GUO 23 Dec '20

23 Dec '20

I finished earlier. Feel free to use Best, Boyu Boyu Guo - Predoctoral fellow Brassinosteriods VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 34 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] LSM710 free until 12h
by Anchal Bisht 23 Dec '20

23 Dec '20

Anchal Bisht - Predoctoral fellow Cell Cycle VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 64 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 free from 19h till 21h
by Jonas DE BACKER 22 Dec '20

22 Dec '20

Feel free to take it Jonas De Backer - Predoctoral fellow Oxidative Stress Signaling VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 33 92 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Olympus free within 30 minutess, feel fre to use
by Evelien Mylle 21 Dec '20

21 Dec '20

Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 free till 11h
by Lucas ALVES NEUBUS CLAUS 21 Dec '20

21 Dec '20

Lucas Alves Neubus Claus - Predoctoral fellow Brassinosteriods VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 36 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SD: is someone planning to use the dual camera setup for the coming weeks?
by Evelien Mylle 18 Dec '20

18 Dec '20

Dear spinning disc users, Is someone planning to use the dual camera setup for the coming weeks? If so, can you let me know? Thanks, Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Zeiss 710: stitching and macro editing possible (2 licenses that are transferred from the Zeiss Exciter)
by Evelien Mylle 16 Dec '20

16 Dec '20

Dear Zeiss 710 users, This confocal has from today also the possibility to perform stitching and to edit Macros , both tools that were only possible on the Zeiss Exciter confocal before. When selecting 'Tile scan' the option 'overlap' is now usable, allowing to stitch the images and not only tile the images (= putting next to each other). In case of questions, don't hesitate to contact Daniel or me. Enjoy!! Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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