February 2020 - confocal@psb.ugent.be

[Confocal] SD: dichroic exchanged for simultaneously imaging CFP/YFP, instead of GFP/RFP
by Evelien Mylle 23 Feb '21

23 Feb '21

Dear all, The 509 beamsplitter is now installed allowing to simultaneously image CFP/YFP. If you would like to simultaneously image GFP/RFP the beamsplitter needs to be changed to the 580 beamsplitter. This is physically taking out the filter and put the other one in, something that needs to be done by Daniel or me. Keep in mind that before using both cameras you ALWAYS need to calibrate first. This can be done through a userfriendly wizard 'Calibrate Multicamera' (under Video). If not clear, don't hesitate to ask for help. Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SD: issue with rear camera, cameras exchanged, please contact me when you will use it
by Evelien Mylle 13 Mar '20

13 Mar '20

Dear all, The cameras of the spinning disc are exchanged as the rear camera (the mostly used one) gives a very pixeled image. The top camera is now on the place of the rear camera, but settings are for the moment still as top camera. With some adaptations you can use it as normal. Please contact me if you cant to use it, so I can help you with this. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8X Argon laser
by Daniel Van Damme 28 Feb '20

28 Feb '20

Dear all, The Argon laser of the Leica SP8X is getting unstable. This has nothing to do with the power break. You can still use it, but you have to restrict it to 20% in the window where you activate it. Running the Argon laser at low output will anyway increase its life span, so from now on, this should be implemented at all times. have fun imaging, d Prof. Dr. ir. Daniel Van Damme - Group leader Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 70 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP2 user
by Alaeddine SAFI 27 Feb '20

27 Feb '20

who is using the SP2? It's on since I arrived at 18h and nobody shows up.. Alaeddine Safi - Postdoctoral fellow Root Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 38 68 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Spinning disk free from 2pm today
by Jonathan DRAGWIDGE 27 Feb '20

27 Feb '20

Spinning disk free from 2pm today, feel free to use it. Jonathan Dragwidge - Postdoctoral fellow Advanced Live Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 22 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8 free today from 15:40 - 17:00
by Lucas ALVES NEUBUS CLAUS 25 Feb '20

25 Feb '20

Feel free to use it. Lucas Alves Neubus Claus - Predoctoral fellow Brassinosteriods VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 36 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] 710 free 9.30 to 12.00
by Alvaro Fernandez 25 Feb '20

25 Feb '20

Feel free to take it. Álvaro Alvaro Daniel Fernandez Fernandez - Predoctoral fellow Oxidative Stress Signalling VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 22 www.psb.ugent.be Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8 free 9 - 13 00
by Darja STOJANOVOVA 25 Feb '20

25 Feb '20

Dear Confocal users, The Leica SP8 is free this morning till 1 pm. Feel free to use. Darja Stojanovova - Predoctoral fellow Cell Cycle VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 36 93 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] test from IT
by Dany Cuyt 24 Feb '20

24 Feb '20

-- Dany Cuyt - System & Security engineer I.T. Support VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 38 17 www.psb.ugent.be Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] test
by Evelien Mylle 24 Feb '20

24 Feb '20

Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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