March 2020 - confocal@psb.ugent.be - maillist.psb.ugent.be

[Confocal] SD: dichroic exchanged for simultaneously imaging CFP/YFP, instead of GFP/RFP
by Evelien Mylle 23 Feb '21

23 Feb '21

Dear all, The 509 beamsplitter is now installed allowing to simultaneously image CFP/YFP. If you would like to simultaneously image GFP/RFP the beamsplitter needs to be changed to the 580 beamsplitter. This is physically taking out the filter and put the other one in, something that needs to be done by Daniel or me. Keep in mind that before using both cameras you ALWAYS need to calibrate first. This can be done through a userfriendly wizard 'Calibrate Multicamera' (under Video). If not clear, don't hesitate to ask for help. Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8X
by Daniel Van Damme 17 Jun '20

17 Jun '20

Dear confocal users, The cooling system on the Leica leaks. The piece has been ordered and hopefully will arrive soon. If all goes well, it should be here next Monday (16/3). For now, we took the cooling box out to be able to monitor it. There is a red line which marks the lowest possible liquid level. When the liquid level reaches this point, contact me or Evelien. Failure to cool will render the system unstable. Also, the confocal box is open, so whenever you image, be careful not to push it. This is the only option to keep imaging until we have a repair unit. Also, no imaging is allowed before 9am or after 19h and also not in the weekend as there is no way to monitor this safely in the evening/night If you have questions, contact Evelien or me. cheers, d Prof. Dr. ir. Daniel Van Damme - Group leader Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 70 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 free this morning, except for Leica intervention/
by Jos Wendrich 16 Mar '20

16 Mar '20

Due to last minute changes in lab-opening I have to cancel my booking for this morning. Feel free to use. Jos Jos Wendrich, PhD Post-Doc PSB, VIB-UGent Vascular Development Technologiepark Zwijnaarde 71 9052 Ghent, Belgium Email: jos.wendrich(a)psb.ugent.be Phone: +32 9 331 39 43 http://www.psb.ugent.be/vascular-development Google Scholar: https://scholar.google.nl/citations?user=Cqs0p58AAAAJ&hl=nl ORCID: https://orcid.org/0000-0002-6198-3763 Research Gate: https://www.researchgate.net/profile/Jos_Wendrich LinkedIn: https://nl.linkedin.com/in/jos-wendrich-29642416

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[Confocal] Please adapt your confocal reservations!
by Evelien Mylle 16 Mar '20

16 Mar '20

Dear all, Would it be possible to adapt your confocal reservation or at least send an email informing the other users? On Friday a lot of systems kept running without being used. Thanks in advance, Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SD: issue with rear camera, cameras exchanged, please contact me when you will use it
by Evelien Mylle 13 Mar '20

13 Mar '20

Dear all, The cameras of the spinning disc are exchanged as the rear camera (the mostly used one) gives a very pixeled image. The top camera is now on the place of the rear camera, but settings are for the moment still as top camera. With some adaptations you can use it as normal. Please contact me if you cant to use it, so I can help you with this. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] FLIM laser not working
by Evelien Mylle 10 Mar '20

10 Mar '20

Dear FLIM users, The control box for the FLIM laser is not working and send to the company for repair. Return is expected within 3 weeks. I will keep you updated. Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 free 14:00-17:00 (03/12) This Thursday
by Baojun Yang 10 Mar '20

10 Mar '20

Feel free to use! Baojun Baojun Yang - Postdoctoral fellow Vascular Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 43 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8: thursday whole day intervention
by Evelien Mylle 10 Mar '20

10 Mar '20

Dear all, There will be a whole day intervention on the SP8 from Leica this thursday (12/03). Please keep this in mind for your planned experiments. I could not register this in the calendar because of existing reservations. In case you cancel, let me know than I can reserve this maintenance. @ Peter: you could use your session till the person arrives. Arrival time is depending on the traffic. @ Xiapeng: I assume your session will not be influenced, but just check a bit in advance. Sorry for the inconvenience, Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 free today until 15h
by Jos Wendrich 09 Mar '20

09 Mar '20

Sorry again, but my samples were crap so no use in imaging them.. feel free to use! Cheers, Jos PS: WLL and fluorescent lamp were turned off just 35min ago, keep this in mind when using it. Jos Wendrich, PhD Post-Doc PSB, VIB-UGent Vascular Development Technologiepark Zwijnaarde 71 9052 Ghent, Belgium Email: jos.wendrich(a)psb.ugent.be Phone: +32 9 331 39 43 http://www.psb.ugent.be/vascular-development Google Scholar: https://scholar.google.nl/citations?user=Cqs0p58AAAAJ&hl=nl Research Gate: https://www.researchgate.net/profile/Jos_Wendrich LinkedIn: https://nl.linkedin.com/in/jos-wendrich-29642416

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[Confocal] Sp8: this Friday morning intervention
by evmyl＠psb.vib-ugent.be 06 Mar '20

06 Mar '20

Dear sp8 users,This Friday morning there will be intervention from Leica. I estimate that this would take around 1 hour, starting at 9.However, this is estimation, so changes can happen. Please keep this in mind for your experiments.Gr,Evelien Verzonden vanaf mijn Samsung Galaxy-smartphone.

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