May 2020 - confocal@psb.ugent.be - maillist.psb.ugent.be

[Confocal] SD: dichroic exchanged for simultaneously imaging CFP/YFP, instead of GFP/RFP
by Evelien Mylle 23 Feb '21

23 Feb '21

Dear all, The 509 beamsplitter is now installed allowing to simultaneously image CFP/YFP. If you would like to simultaneously image GFP/RFP the beamsplitter needs to be changed to the 580 beamsplitter. This is physically taking out the filter and put the other one in, something that needs to be done by Daniel or me. Keep in mind that before using both cameras you ALWAYS need to calibrate first. This can be done through a userfriendly wizard 'Calibrate Multicamera' (under Video). If not clear, don't hesitate to ask for help. Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8X
by Daniel Van Damme 17 Jun '20

17 Jun '20

Dear confocal users, The cooling system on the Leica leaks. The piece has been ordered and hopefully will arrive soon. If all goes well, it should be here next Monday (16/3). For now, we took the cooling box out to be able to monitor it. There is a red line which marks the lowest possible liquid level. When the liquid level reaches this point, contact me or Evelien. Failure to cool will render the system unstable. Also, the confocal box is open, so whenever you image, be careful not to push it. This is the only option to keep imaging until we have a repair unit. Also, no imaging is allowed before 9am or after 19h and also not in the weekend as there is no way to monitor this safely in the evening/night If you have questions, contact Evelien or me. cheers, d Prof. Dr. ir. Daniel Van Damme - Group leader Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 70 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 reservation from 17:00-24:00 on 25/05 cancelled
by Qing Lu 15 May '20

15 May '20

Sorry for any inconvenience Qing Lu - Predoctoral fellow Brassinosteroids VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 36 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8X free
by Balkan Canher 14 May '20

14 May '20

I won't need the rest of my reservation because my sample is not good. Feel free to use it. If nobody replies in 30min I will turn it off. Greetings Balkan Canher - Postdoctoral fellow Cell Cycle VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Argon laser on the SP8X not working properly
by Balkan Canher 04 May '20

04 May '20

Dear All, The Argon laser on the SP8X was not working properly last time I used it (01/05). Especially at higher laser power setting, the laser fails to continuously emit. Maybe the laser is nearing retirement? Regards, Balkan Canher - Predoctoral fellow Cell Cycle VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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