July 2020 - confocal@psb.ugent.be - maillist.psb.ugent.be

[Confocal] SD: dichroic exchanged for simultaneously imaging CFP/YFP, instead of GFP/RFP
by Evelien Mylle 23 Feb '21

23 Feb '21

Dear all, The 509 beamsplitter is now installed allowing to simultaneously image CFP/YFP. If you would like to simultaneously image GFP/RFP the beamsplitter needs to be changed to the 580 beamsplitter. This is physically taking out the filter and put the other one in, something that needs to be done by Daniel or me. Keep in mind that before using both cameras you ALWAYS need to calibrate first. This can be done through a userfriendly wizard 'Calibrate Multicamera' (under Video). If not clear, don't hesitate to ask for help. Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8 free until 17h!
by Joris JOURQUIN 31 Jul '20

31 Jul '20

Feel free to take it! Joris Joris Jourquin - Predoctoral fellow Root Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 38 46 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] on holiday from 31/07 till 23/08: please contact Daniel in case of problems
by Evelien Mylle 30 Jul '20

30 Jul '20

Dear confocal users, In case of confocal problems, you can contact Daniel. (from Monday 03/08). Please put me in cc, so that I'm up to date on the ongoing problems. As of 24/08 I'm back at your disposal. I wish you all a nice summer! All the best, Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8: firmware event code
by Evelien Mylle 30 Jul '20

30 Jul '20

Dear SP8 users, We have again on regular base following error. A restart (only software of if it doesn't help a complete restart) is the only solution for the moment. The company checked our PC but for the moment there is nothing that could be linked to the cause of this. To investigate this further could you give me more details when you observe this error? Please do the following: - create error report (indicated on a sheet at the wall next to the SP8) - save on [ file://psb.ugent.be/shares/research/images/images_cameras/confocal/Problems_Leica%20SP8%20-- | \\psb.ugent.be\shares\research\images\images_cameras\confocal\Problems_Leica SP8 -- ] > make new folder with date and mention firmware event code - also add a document which mentions where your project was saved on the moment of the error - send me an email I also would like to remind you to following procedure. It could help to prevent above error (not sure), but anyway, it's a better way of working as the machine will be faster. Enjoy! Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] sp8: bidirectional scanning and speed
by Evelien Mylle 30 Jul '20

30 Jul '20

Dear SP8 users, I would like to draw your attention to the use of bidirectional scanning and the link to the speed. The use of bidirectional scanning requires a correction factor (phase factor), which depends on your speed. Default is that value set for a speed of 400 or 600. At higher or lower speed, the correction will not be good, resulting in a bad image. Of course you can adjust this yourself until it seems good. Leica can determine the optimal correction factor for each speed, but only 1 value can be entered to be used as default. If there would be a demand for this, I can request to have these other values, which can be insterted manual when you need it (but at least you know the correct factor and don't have to search for it). I can also choose another speed as default value. Please let me know if you are interested and I can arrange this during an intervention. For your information: imaging at a speed of 700, no bidirectional scanning requires for a certain setup 1.49 s. Using a speed of 600, bidirectional scanning needs for the same setup 871 ms. Speed also influences the amount of signal you get. Below some print screens with info about speed, bidirectional and some pictures. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Olympus confocal free today from 17:30; tomorrow whole day free
by Evelien Mylle 28 Jul '20

28 Jul '20

Dear all, Feel free to use the olympus confocal today from 17:30. Also the whole day tomorrow the confocal is free. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 free today from 14-17
by Peter GRONES 20 Jul '20

20 Jul '20

Hi, SP8 is free today from 2pm-5pm. My experiment did not work. Feel free to use Greetings, Peter Peter Grones - Postdoctoral fellow Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 73 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 free TODAY from 17:00 to 20:00
by Astrid Gadeyne 17 Jul '20

17 Jul '20

Cheers, Astrid Astrid Gadeyne - Postdoctoral fellow [ https://www.psb.ugent.be/functional-interactomics | Functional Interactomics ] [ https://www.psb.ugent.be/functional-interactomics ] VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 38 73 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8 free 14:00 - 17:00
by Darja STOJANOVOVA 16 Jul '20

16 Jul '20

Leica is free today 14:00 - 17:00 Cheers, Darja Darja Stojanovova - Predoctoral fellow Cell Cycle VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 36 93 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Olympus FV10 free 16/07 thursday
by Balkan Canher 16 Jul '20

16 Jul '20

feel free to take it Cheers Balkan Canher - Postdoctoral fellow Cell Cycle VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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