September 2020 - confocal@psb.ugent.be

[Confocal] SD: dichroic exchanged for simultaneously imaging CFP/YFP, instead of GFP/RFP
by Evelien Mylle 23 Feb '21

23 Feb '21

Dear all, The 509 beamsplitter is now installed allowing to simultaneously image CFP/YFP. If you would like to simultaneously image GFP/RFP the beamsplitter needs to be changed to the 580 beamsplitter. This is physically taking out the filter and put the other one in, something that needs to be done by Daniel or me. Keep in mind that before using both cameras you ALWAYS need to calibrate first. This can be done through a userfriendly wizard 'Calibrate Multicamera' (under Video). If not clear, don't hesitate to ask for help. Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SD: other joystick, no dual camera, please report problems from coming weeks
by Evelien Mylle 27 Nov '20

27 Nov '20

Dear spinning disc users, Here are some temporary changes to our spinning disc setup: - other joystick - No dual camera setup possible These adjustments are the result of installation of the loan proscan unit. The purpose is to check if the crashes of the volocity software are due to our proscan unit or not. So it is very important to know how the system will behave in the coming period. So PLEASE report and register ALL PROBLEMS ( print screen, session error log, ....)! And inform me about it. As usual you can save this under \psb.ugent.be\shares\research\images_cameras\spinning disc\Problems Spinning disc I will keep you updated about any change. Hoping that the dual camera setup will soon be available. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8: waterpump not working for the moment
by Evelien Mylle 08 Oct '20

08 Oct '20

Dear SP8 users, The waterpump is ifor the moment not available. Our help is looking into it. Hoping that this ill be repaired by the end of the week. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] FV10 Olympus free Thursday
by Balkan Canher 30 Sep '20

30 Sep '20

I deleted my reservation for the FV10 for Thursday. Feel free to take it. Cheers Balkan Canher - Postdoctoral fellow Cell Cycle VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Olympus confocal free tomorrow whole day
by Evelien Mylle 29 Sep '20

29 Sep '20

Dear Olympus users, The olympus confocal is tomorrow free whole day. The sheduled maintenance is cancelled. I cancelled the reservation. Feel free to reserve. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 free till 20:00
by Jonas DE BACKER 28 Sep '20

28 Sep '20

I finished my experiment earlier so the SP8 is free till 20:00. Feel free to use it. Kind regards, Jonas De Backer Jonas De Backer - Predoctoral fellow Oxidative Stress Signaling VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 33 92 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Sp8 free on Wednesday 10-17.
by Joanna Winkler 28 Sep '20

28 Sep '20

Feel free to take it. Greetings, Joanna Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 73 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 free until 12.00
by Alvaro Fernandez 28 Sep '20

28 Sep '20

Enjoy imaging, Alvaro Alvaro Daniel Fernandez Fernandez - Predoctoral fellow Oxidative Stress Signalling VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 22 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 cancelled on Friday 25/09, 18:00-21:00, feel free to use
by Baojun Yang 24 Sep '20

24 Sep '20

Baojun Yang - Postdoctoral fellow Vascular Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 43 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8 free tomorrow from 10-13
by Peter GRONES 24 Sep '20

24 Sep '20

Feel free to use it. Peter Peter Grones - Postdoctoral fellow Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 73 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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