January 2021 - confocal@psb.ugent.be

[Confocal] SD: dichroic exchanged for simultaneously imaging CFP/YFP, instead of GFP/RFP
by Evelien Mylle 23 Feb '21

23 Feb '21

Dear all, The 509 beamsplitter is now installed allowing to simultaneously image CFP/YFP. If you would like to simultaneously image GFP/RFP the beamsplitter needs to be changed to the 580 beamsplitter. This is physically taking out the filter and put the other one in, something that needs to be done by Daniel or me. Keep in mind that before using both cameras you ALWAYS need to calibrate first. This can be done through a userfriendly wizard 'Calibrate Multicamera' (under Video). If not clear, don't hesitate to ask for help. Best regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica sp8 is free
by Joanna Winkler 29 Jan '21

29 Jan '21

Feel free to take it. Greetings Joanna Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 73 www.psb.ugent.be Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SP8 free now until 18:00
by Matous GLANC 27 Jan '21

27 Jan '21

finished a little earlier, enjoy! Matouš Glanc - Postdoctoral fellow Vascular Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 45 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] zeiss 710 reservation cancelled
by Sylwia Struk 27 Jan '21

27 Jan '21

on the 29th of January 17:00-19:00 and the 1st of February 9:00-12:00. Best, Sylwia Sylwia Struk - Postdoctoral fellow Rhizosphere VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 06 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Zeiss 710 free today 17:30-19:00 & 20:30-21:30
by Fei XIE 27 Jan '21

27 Jan '21

Fei XIE - Predoctoral fellow Programmed Cell Death VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 16 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] test confocal mail list
by Toon MERTENS 26 Jan '21

26 Jan '21

Dear, This is a test email to the confocal mail list. Kind regards, Toon Toon Mertens - Predoctoral fellow [ https://www.psb.ugent.be/functional-interactomics | Functional Interactomics ] [ https://www.psb.ugent.be/functional-interactomics ] VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 38 75 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] SD: upgraded to windows 10, some minor changes for the users
by Evelien Mylle 26 Jan '21

26 Jan '21

Dear all, The spinning disc has been upgraded to Windows 10 . The differences for working on the spinning disc are very minor: - During the startup of the PC you have to confirm by clicking any key . Without doing this, the PC will be waiting and not startup completely - Log in to PC as psbuser (password is on a note on the screen). So, no automatic login anymore - Within the software there could be some minor changes as icons that are in another order (f.ex. lightpath for the rear camera starts now with the R, than followed by 405, ... whereas the R was in Windows 7 more at the end of the row) For the moment, I didn't observed any issues with the software under Windows 10. However I didn't do much of imaging. So, please, inform Daniel or me, in case you have issues. We should know if this system works well under Windows 10. Thanks in advance, Kind regards, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Zeiss 710 free till 9:30
by Peter GRONES 26 Jan '21

26 Jan '21

Hi, I finished my experiment earlier today. Greetings, Peter Peter Grones - Postdoctoral fellow Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 73 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica sp8 free today 12:00-18:30
by Joanna Winkler 25 Jan '21

25 Jan '21

Feel free to use it. Greetings, Joanna Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 73 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8 free on Thursday (28th) morning
by Peter GRONES 25 Jan '21

25 Jan '21

Feel free to use it. Greetings, Peter Peter Grones - Postdoctoral fellow Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 73 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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