- confocal@psb.ugent.be - maillist.psb.ugent.be

Re: [Confocal] did someone use the exciter during the week-end?
by heboi 24 Oct '11

24 Oct '11

Problem solved. Please once again follow the procedure when switching off and on the confocals. 405 lamp has been on all the week-end. And I lost 1 hour of my time because of a stupid mistake during a switch off procedure. So thanks to make me fell good on a monday morning !!! Helene On Mon, 24 Oct 2011 09:26:29 +0200, Claudiu Niculaes <clnic(a)psb.vib-ugent.be> wrote: > On 10/24/2011 9:16 AM, heboi wrote: >> Please conatct me. >> Helene > I used it on Friday, but there were others after me. Did something > happen to it?

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Re: [Confocal] did someone use the exciter during the week-end?
by heboi 24 Oct '11

24 Oct '11

The microscope of the Exciter 5 does not respond. No power and no connexion to the computer. Right now the confocal is out of use. If someone as a clue how to solve it, I am in the confocal room. Helene On Mon, 24 Oct 2011 09:26:29 +0200, Claudiu Niculaes <clnic(a)psb.vib-ugent.be> wrote: > On 10/24/2011 9:16 AM, heboi wrote: >> Please conatct me. >> Helene > I used it on Friday, but there were others after me. Did something > happen to it?

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[Confocal] Exciter is free from 10 to 12
by Yuliya Salanenka 21 Oct '11

21 Oct '11

i am canceling my session, please take it over! tnx, yuliya -- ================================================================== Yuliya Salanenka Tel:+32 (0)9 331 38 65 fax:+32 (0)9 3313809 VIB Department of Plant Systems Biology, Ghent University Technologiepark 927, 9052 Gent, BELGIUM yusal(a)psb.vib-ugent.be http://www.psb.vib-ugent.be ==================================================================

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[Confocal] old zeiss free
by Wim Grunewald 19 Oct '11

19 Oct '11

-- ================================================================== Wim Grunewald AUXIN lab VIB Department of Plant Systems Biology, Ghent University Technologiepark 927, 9052 Gent, BELGIUM Tel:+32 (0)9 331 39 15 fax:+32 (0)9 331 38 09 http://www.psb.vib-ugent.be ==================================================================

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[Confocal] zeiss 710 free from now until 20h
by Daniel Van Damme 18 Oct '11

18 Oct '11

-- ================================================================== Daniel Van Damme Tel:+32 (0)9 331 39 33 fax:+32 (0)9 3313809 VIB Department of Plant Systems Biology, Ghent University Technologiepark 927, 9052 Gent, BELGIUM dadam(a)psb.vib-ugent.be http://www.psb.vib-ugent.be ==================================================================

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[Confocal] NEW ZEISS free from 17 to 20 - tomorrow
by kabla 17 Oct '11

17 Oct '11

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[Confocal] Exciter is free already
by Yuliya Salanenka 17 Oct '11

17 Oct '11

I am done already, the follower could start earlier. cheers, yuliya -- ================================================================== Yuliya Salanenka Tel:+32 (0)9 331 38 65 fax:+32 (0)9 3313809 VIB Department of Plant Systems Biology, Ghent University Technologiepark 927, 9052 Gent, BELGIUM yusal(a)psb.vib-ugent.be http://www.psb.vib-ugent.be ==================================================================

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[Confocal] Confocal update
by Daniel Van Damme 07 Oct '11

07 Oct '11

Dear all, The Olympus confocal is up and running (at least for the moment). The laser output (488) is 25mW instead of 35mW, but this should be usable. A new laser has been ordered and will be installed in the coming weeks. It is probable that the laser has an ignition problem, so if it does not start, give it some time. It does not harm to keep it on for a long time before it ignites. If it still refuses to ignite, contact me. Regarding the multi-area timelapse, the system jammed, probably because somebody was saving images on a personal drive. If another person subsequently logs in, the system tries to access the previous drive location and as it does not get access, the system crashes. Please only use the Olympus drive to store your images as normally everybody who uses the confocals should have access to this drive. to solve the problem, we generated another user account: ID: GUEST password: guest With this account, the multi-area timelapse module works again, it does not work with the previous account. I would also like to stress that people who are not familiar with the microscopes should get training or at least ask for help. This morning, the Zeiss710 was down and it took Lukasz, Matthias and me more than half an hour to finally figure out that somebody has been turning the key on the main control box. Please follow the normal procedure when switching off the microscopes and do not turn off any other switches. Have fun imaging, Daniel -- ================================================================== Daniel Van Damme Tel:+32 (0)9 331 39 33 fax:+32 (0)9 3313809 VIB Department of Plant Systems Biology, Ghent University Technologiepark 927, 9052 Gent, BELGIUM dadam(a)psb.vib-ugent.be http://www.psb.vib-ugent.be ==================================================================

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[Confocal] Fwd: Reservation deleted for 10/04/2011, 14-18hr, Exciter
by Helene Boisivon 03 Oct '11

03 Oct '11

-- Helene Robert-Boisivon Auxin, Polarity, Patterning Department of Plant Systems Biology VIB-Ghent University Technologiepark 927 9052 Ghent Belgium Tel 32 (0) 9 3313915 Fax 32 (0) 9 3313809 http://www.psb.vib-ugent.be helene.boisivon(a)psb.vib-ugent.be Begin forwarded message: > From: "PSB planner" <help(a)psb.vib-ugent.be> > Date: 3 October 2011 11:26:11 PM GMT+02:00 > To: "Helene" <heboi(a)psb.vib-ugent.be> > Cc: "Helene" <heboi(a)psb.vib-ugent.be> > Subject: Reservation deleted for 10/04/2011 > > Helene, > You have successfully deleted reservation #sc14e7c9fdb8b206. > > Please use this reservation number when contacting the administrator > with any questions. > > A reservation between 10/04/2011 14:00 and 10/04/2011 18:30 for > Zeiss LSM5 Exiter confocal located at E142 has been deleted. > > If this is a mistake, please contact the administrator at: help(a)psb.ugent.be > or by calling 13915. > > You can view or modify your reservation information at any time by > logging into PSB planner at: > https://intra3.psb.ugent.be/psbplanner/. > > Please direct all technical questions to help(a)psb.ugent.be. > > + > ----------------------------------------------------------------------------------------------------------------------+ > | Reservation > deleted > | > + > ----------------------------------------------------------------------------------------------------------------------+ > | Reservation # | Start Date | End Date | > Resource | Start Time | End Time | Location | > Contact | > + > ----------------------------------------------------------------------------------------------------------------------+ > | sc14e7c9fdb8b206 | 10/04/2011 | 10/04/2011 | Zeiss LSM5 Exiter > confocal | 14:00 | 18:30 | E142 | 13915 | > + > ----------------------------------------------------------------------------------------------------------------------+ > >

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[Confocal] FV1000 multi area timelapse
by Daniel Van Damme 29 Sep '11

29 Sep '11

Dear all, The multi-timelapse application of the FV1000 is corrupted and cannot be used for the moment. Otherwise, the system is operational but you are restricted to a single point timelapse. I have contacted the people from Olympus to take a look at this and I will inform you of the progress. Greetings, Daniel -- ================================================================== Daniel Van Damme Tel:+32 (0)9 331 39 33 fax:+32 (0)9 3313809 VIB Department of Plant Systems Biology, Ghent University Technologiepark 927, 9052 Gent, BELGIUM dadam(a)psb.vib-ugent.be http://www.psb.vib-ugent.be ==================================================================

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