- confocal@psb.ugent.be - maillist.psb.ugent.be

[Confocal] Leica SP8 free from 13 - 18:00 tomorrow 13/11
by Lucas ALVES NEUBUS CLAUS 12 Nov '19

12 Nov '19

Please feel free to use Lucas Alves Neubus Claus - Predoctoral fellow Brassinosteriods VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 36 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Zeiss Exciter: CH1 not working, FRAME sequential imaging can be used
by Evelien Mylle 08 Nov '19

08 Nov '19

Dear all, The Ch1 from the Zeiss Exciter is not working anymore. This influences mainly the 2-colour imaging , and mainly your red dye or fluorophore. One colour imaging can be done by sending everything to the first detector (Ch2), meaning that reuse of the settings of a previous image could need some adaptation. Two colour imaging could be done by FRAME sequential imaging : both your dyes will be captured at the same channel, but one after the other. Keep in mind that there is more time-delay between imaging your 2 colours compared to the simultanous or line sequential mode, which is normally used. During live mode I would recommand to only use one channel at a time as it takes more time to see the effect in the second channel. This can be easily done in the software by activating-deactivating a channel (also explained in the ppt in attach). More information in attach and also in the help folder, including a picture with settings allowing to reload the settings for GFP - PI FRAME sequential imaging: S:\research\images\images_cameras\confocal\Help Zeiss Exciter\20191108_Frame sequential scanning GFP PI\ GFP and PI_Frame sequential.lsm Don't hesitate to contact Daniel or me if you would have problems with the lightpath setup. I will keep you update about the repair of this channel. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica sp8 free until 12 today
by Joanna Winkler 07 Nov '19

07 Nov '19

Greetings, Joanna Joanna Winkler - Predoctoral fellow Advanced Live Cell Imaging,Functional Interactomics VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 73 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leice SP8 Free today overnight and tomorrow overnight
by Balkan Canher 07 Nov '19

07 Nov '19

Dear All I won't be able to use my reservations with Leice SP8 Free today overnight and tomorrow overnight. Feel Free to take it. Kind Regards, Balkan Canher - Predoctoral fellow Cell Cycle VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 33 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8 free from 14 - 18:00 this afternoon
by Darja STOJANOVOVA 04 Nov '19

04 Nov '19

Please feel free to use Darja Stojanovova - Predoctoral fellow Cell Cycle VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 36 93 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] LSM710 free today 12-13
by Matous GLANC 04 Nov '19

04 Nov '19

Matous Glanc - Postdoctoral fellow Vascular Development VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 45 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] Leica SP8: please inform me if we still have errors
by Evelien Mylle 25 Oct '19

25 Oct '19

Dear all, Could you please inform me and register if you still would encounter errors with the LasXsoftware? The upgrade of the software has been done, hoping that most of the issues we had will be solved with this. If not, please inform me. Also register the problem with the error report (LasX software - Help- Create error report), take a printscreen of the error, .... Please save it all on S:\research\images\images_cameras\confocal\Problems_Leica SP8\ (make subfolder with data and name) Hoping that you can enjoy a relaxing imaging time on the SP8!! Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] tomorrow intervention from Leica SP8, for sure whole morning
by Evelien Mylle 24 Oct '19

24 Oct '19

Dear all, Tomorrow we will have an intervention from Leica SP8. They will start probably around 08:30u and will for sure need the morning. The upgrade of the software will be done, hoping that part of the software errors would be solved with that. (not sure if this will be the case, but this is the first step to do. Looking into these software errors only makes sense with an upgraded software.) Also the repair part for the mirrors for the microscope will hopefully be installed tomorrow (if transport goes according to shedule). I know this is very short notice and this will interfere with your experiments, which I really regret. But I don't see another option. I've delayed this software upgrade already a lot as I wanted to shedule this without interfering the users, but this is a challenging task. Sometimes last-minutes intervention pops up if the Leica people need to be at the IRC site, and they try to combine this with an intervention at our place, which is now the case. Please realize that we are out of guarantee period and we need to pay for each intervention, including transport. A combined visit with IRC is for us also interesting. Lucas, Isabelle, Klaas, Most probably your reservation tomorrow could be taken by the intervention, depending on how it goes. I keep you update. Gr, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] LSM5 free tomorrow morning
by Anchal Bisht 24 Oct '19

24 Oct '19

Feel free to use it ! Anchal Bisht - Predoctoral fellow Cell Cycle VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 64 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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[Confocal] who used the Zeiss Exciter yesterday evening?
by Evelien Mylle 23 Oct '19

23 Oct '19

Dear all, I'm looking for the person who used the Zeiss Exciter yesterday evening (after the use of Anchal)? All info is wellcome! If you are the one, could you please contact me? Thanks, Evelien Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ] Genome editing, cutting-edge technology for a sustainable agriculture

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