Dear all, The output of the argon laser on Olympus is down to 20%. This means that it has reached its end phase and it will probably not last much longer. Please be aware that you will need to use substantially more laser % when imaging with this laser as the output is down. Also, all other lasers do not need to be left on when you finish, it is only the argon laser which is giving problems igniting which is why we leave it on for the time being. the diode lasers should be turned off whenever not used. I will try to get this laser replaced as soon as possible. Greetings, Daniel -- ================================================================== Daniel Van Damme Tel:+32 (0)9 331 39 33 fax:+32 (0)9 3313809 VIB Department of Plant Systems Biology, Ghent University Technologiepark 927, 9052 Gent, BELGIUM dadam@psb.vib-ugent.be http://www.psb.vib-ugent.be ==================================================================