Hi Jelle,

thank you for letting us know! I checked now, the argon laser is in principle on, but the 488nm emission is very, very weak (barely visible by eye at 100%) and I have not yet been able to switch to 515nm.

I have not yet started the experiment, but seeing the emission, I am not even sure I will, if I get any result I will let you know.

I think it is pretty safe to say the argon laser is in a terminal coma, if not already gone :(

Matous

Matouš Glanc - Postdoctoral fellow
Vascular Development

VIB-UGent Center for Plant Systems Biology
Ghent University
Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium
Tel. +32(0)9 331 39 45
www.psb.ugent.be

Genome editing, cutting-edge technology for a sustainable agriculture


From: "Jelle Van Leene" <Jelle.VanLeene@psb.ugent.be>
To: "confocal" <confocal@psb.ugent.be>
Cc: "Matous GLANC" <matous.glanc@psb.vib-ugent.be>, "Jonah Nolf" <jonol@psb.ugent.be>, "masim" <masim@psb.vib-ugent.be>, "Anchal Bisht" <anbis@psb.ugent.be>, "Ignacio Achon" <igach@psb.vib-ugent.be>, "Lana Hellebaut" <lahel@psb.vib-ugent.be>
Sent: Monday, November 16, 2020 8:03:01 PM
Subject: Fwd: [Confocal] olympus confocal: IMPORTANT!! switching off adapted        procedure!!!

Hi all

I wanted to use the Olympus today, however the argon laser was off this morning and I wasn't able to activate the laser again...
I left the laser on overnight so hopefully it will be active again by tomorrow.

fingers crossed!
Jelle

From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be>
To: Confocal@psb.vib-ugent.be, "Ignacio Achon" <igach@psb.vib-ugent.be>
Sent: Friday, 30 October, 2020 16:39:44
Subject: [Confocal] olympus confocal: IMPORTANT!! switching off adapted        procedure!!!
Dear Olympus users,
@ Ignachio, can you confirm as you are the last user today. (I could not find you in the lab)

Please follow the instructions in picture below or in attach in order to switch ONLY the 559 and fluorescence lamp off, so LEAVING THE ARGON LASER ON!! 

Reason is the following: 
The argon laser took this morning more than one hour to get properly working.   
THis, together with the non -working 515 laser, makes me thinks that the argon laser is at his end.  
From experience, not getting the laser on is often one of the first signs.  Once it's on, it's all OK. 
That's why I adjust the switching off procedure by leaving the laser on!  It is a bit risky, with a possible lockdown in prospect, but I don't want to take the risk of not being able to activate the laser on Monday.  The reservations are full for next week, and possibly these are experiments that will be finalized next week.

Do switch off the 559 and the  fluorescence lamp!  
See cadaped switching off procedure below: 


Gr,
Evelien


Gr,

Evelien

Evelien Mylle - Labmanager/ microscopy technician
Advanced Live Cell Imaging

VIB-UGent Center for Plant Systems Biology
Ghent University
Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium
Tel. +32(0)9 331 39 74
www.psb.ugent.be

Genome editing, cutting-edge technology for a sustainable agriculture


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