Dear all, There seems to be a problem with the stage and the mark position software module of the FV1000. It is currently problematic to mark different positions and keep the XYZ stable when moving between the positions, even with the 20x objective. I have contacted Olympus regarding this and I will let you know when it is fixed. Please let me know if anyone else has experienced similar problems with keeping multiple positions in frame when re-registering points for multi area timelapse acquisition. cheers, daniel -- ================================================================== Daniel Van Damme Tel:+32 (0)9 331 39 33 fax:+32 (0)9 3313809 VIB Department of Plant Systems Biology, Ghent University Technologiepark 927, 9052 Gent, BELGIUM dadam@psb.vib-ugent.be http://www.psb.vib-ugent.be ==================================================================