Dear all, The olympus confocal is running at full power again, with a new argon laser. The previous one had 6147 hours, which can be considered a 'beautiful age' and understandable that he needs eternal rest. So, from now on, the procedure is back as before and as described in the manual. In case of problems, please contact Daniel or me. Gr, Evelien
From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be> To: "confocal" <Confocal@psb.vib-ugent.be> Cc: "Ignacio ACHON" <igach@psb.vib-ugent.be>, "Isabelle Van Houtte" <ishou@psb.vib-ugent.be> Sent: Wednesday, November 18, 2020 11:36:12 AM Subject: [Confocal] New argon laser sheduled for 24/11: Re: Olympus confocal: argon laser(458-488 and 515) not working properly
Dear all,
Good news: the new argon laser for the olympus confocal will be installed next Tuesday 24/11. I will let you know when finished.
@ Ignacio: You have a session that day in the morning, but will nog be usable.
Gr,
Evelien
From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be> To: "confocal" <Confocal@psb.vib-ugent.be> Sent: Tuesday, November 17, 2020 9:56:12 AM Subject: [Confocal] Olympus confocal: argon laser(458-488 and 515) VERY VERY VERY weak
Dear olympus confocal users,
The argon laser is giving up its last forces: at the moment there is only a very minimal output, hardly impossible to image something.
The malfunctioning 515 laser, the difficult start up of the argon laser were the first signs of decay. The laser has been ordered for a while, but for now there is no news when it will be delivered and placed.
So now is the ideal time for imaging with the 405 and 559 laser.
I will keep you further informed.
Greetings,
Evelien
From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be> To: "confocal" <Confocal@psb.vib-ugent.be>, "Ignacio ACHON" <igach@psb.vib-ugent.be> Sent: Friday, October 30, 2020 4:39:44 PM Subject: [Confocal] olympus confocal: IMPORTANT!! switching off adapted procedure!!!
Dear Olympus users, @ Ignachio, can you confirm as you are the last user today. (I could not find you in the lab)
Please follow the instructions in picture below or in attach in order to switch ONLY the 559 and fluorescence lamp off, so LEAVING THE ARGON LASER ON!!
Reason is the following: The argon laser took this morning more than one hour to get properly working. THis, together with the non -working 515 laser, makes me thinks that the argon laser is at his end. From experience, not getting the laser on is often one of the first signs. Once it's on, it's all OK. That's why I adjust the switching off procedure by leaving the laser on! It is a bit risky, with a possible lockdown in prospect, but I don't want to take the risk of not being able to activate the laser on Monday. The reservations are full for next week, and possibly these are experiments that will be finalized next week.
Do switch off the 559 and the fluorescence lamp! See cadaped switching off procedure below:
Gr, Evelien
Gr,
Evelien
Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging
VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]
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