Dear all,
The Leica SP8 gives sometimes problems when looking through the microscope to your sample: there is less light and you don't get your sample nicely in focus.
It can be solved by clicking (several times) on live view (in the LAS X software), and/or by switching the lens, and/or switching between DIC and POL light (microscope setting).
Closing and restarting the PC sometimes helps, but not always, and it is very time consuming. So, I would go for the above options.
I assume that there is some kind of filter that gets blocked.
Keep this in mind when doing your imaging especially if you are doing treatments, as it could take a bit more time to set up your imaging.
I will keep you updated.
Gr,
Evelien
Evelien Mylle - Labmanager/ microscopy technician
Advanced Live Cell Imaging
Advanced Live Cell Imaging
VIB-UGent Center for Plant Systems Biology
Ghent University
Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium
Tel. +32(0)9 331 39 74
www.psb.ugent.be
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