[Confocal] SOLVED AGAIN issue with brightfield light Re: update: Leica SP8: sometimes problem with microscope settings

20 Nov 2019

      Dear all, 

The part, causing the recent problems with the brightfield at the microscope, has been replaced. 

Enjoy imaging! 
And as always, in case of problems, please contact Daniel or me. 
Hopefully this will not happen too quickly (or at least not for the SP8). 

Gr, 

Evelien
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From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be>
To: "confocal" <Confocal@psb.vib-ugent.be>
Sent: Monday, November 4, 2019 2:03:31 PM
Subject: [Confocal] AGAIN issue with brightfield light Re: update: Leica SP8:
sometimes problem with microscope settings
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Dear Leica SP8 users,
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We have again the issue with the brightfield light at the microscope that is not
working.
Leica glued the broken part, but I guess that will not have hold very long.
The company will be contacted, and I will keep you up to date as usual.
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For the moment, you could visualize your samples at the microscope either with
fluorescence light or with putting external light above the microscope. (the
lamp is for the moment in use by someone working at the olympus confocal, but
when needed you can take it from there).
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Gr,
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Evelien
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From: "Evelien Mylle" <Evelien.Mylle@psb.ugent.be>
To: "confocal" <Confocal@psb.vib-ugent.be>
Cc: "Daniel Van Damme" <dadam@psb.vib-ugent.be>
Sent: Friday, October 25, 2019 5:24:27 PM
Subject: SOLVED Re: [Confocal] update: Leica SP8: sometimes problem with
microscope settings
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Dear all,
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The broken part has been exchanged and visualizing your samples with brightfield
light at the microscope works again.
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Gr,
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Evelien
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From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be>
To: "confocal" <Confocal@psb.vib-ugent.be>
Sent: Tuesday, October 22, 2019 5:56:38 PM
Subject: Re: [Confocal] update: Leica SP8: sometimes problem with microscope
settings
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Dear all,
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For info: adding extra light (from a normal light source or from mobile phone)
on your sample can help you to visualize your cells on the microscope.
An normal light source is put next to the microscope for this purpose.
Please don't leave it on when you are not there. (to avoid touching the lamp the
microscope).
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Gr,
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Evelien
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From: "Evelien Mylle" <Evelien.Mylle@psb.ugent.be>
To: "confocal" <Confocal@psb.vib-ugent.be>
Cc: "Daniel Van Damme" <dadam@psb.vib-ugent.be>
Sent: Monday, October 21, 2019 12:02:18 PM
Subject: update: Leica SP8: sometimes problem with microscope settings
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Dear all,
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The Leica SP8 can be used again. But without the option to visualize your sample
at the microscope with brightfield. Either fluorescence or directly through
confocal imaging.
However the problem is detected. The sensor to switch the mirrorin the
microscope is broken.
This part will be ordered and renewed. Estimation is that this would be
beginning next week.
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Gr,
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Evelien
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From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be>
To: "confocal" <Confocal@psb.vib-ugent.be>
Sent: Friday, October 18, 2019 5:36:21 PM
Subject: [Confocal] Monday visit Re: Leica SP8: sometimes problem with
microscope settings
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Dear all,
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Leica technician will come on Monday.
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Lucas, Balkan,
Most probably a big part of your session will be used for intervention.
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Gr,
Evelien
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From: "Evelien Mylle" <Evelien.Mylle@psb.ugent.be>
To: Confocal@psb.ugent.be
Cc: "Daniel Van Damme" <dadam@psb.vib-ugent.be>
Sent: Friday, October 18, 2019 4:53:23 PM
Subject: Re: [Confocal] Leica SP8: sometimes problem with microscope settings
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Dear all,
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The problems with visualizing your sample through the microscope on the Leica
SP8 got worse.
We now also have the situation that we have no light at all at the microscope.
Switching between live view (in the LAS X software), and/or by switching the
lens, and/or switching between DIC and POL light (microscope setting), and the
shutters sometimes helps.
Restarting the PC doesn't seem to help much, but you can always try.
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Company will come.
Sorry for this inconvenience,
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Gr,
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Evelien
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> From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be>
> To: "confocal" <Confocal@psb.vib-ugent.be>
> Sent: Friday, August 30, 2019 5:01:07 PM
> Subject: [Confocal] Leica SP8: sometimes problem with microscope settings
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> Dear all,
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> The Leica SP8 gives sometimes problems when looking through the microscope to
> your sample: there is less light and you don't get your sample nicely in focus.
> It can be solved by clicking (several times) on live view (in the LAS X
> software), and/or by switching the lens, and/or switching between DIC and POL
> light (microscope setting).
> Closing and restarting the PC sometimes helps, but not always, and it is very
> time consuming. So, I would go for the above options.
> I assume that there is some kind of filter that gets blocked.
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> Keep this in mind when doing your imaging especially if you are doing
> treatments, as it could take a bit more time to set up your imaging.
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> I will keep you updated.
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> Gr,
> Evelien
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> Evelien Mylle - Labmanager/ microscopy technician
> Advanced Live Cell Imaging
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> VIB-UGent Center for Plant Systems Biology
> Ghent University
> Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium
> Tel. +32(0)9 331 39 74
> [ http://www.psb.ugent.be/ | www.psb.ugent.be ]
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> Genome editing, cutting-edge technology for a sustainable agriculture
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