Dear all,
The Ch1 from the Zeiss Exciter is not working anymore.
This influences mainly the 2-colour imaging, and mainly your red dye or fluorophore.
One colour imaging can be done by sending everything to the first detector (Ch2), meaning that reuse of the settings of a previous image could need some adaptation.
Two colour imaging could be done by FRAME sequential imaging: both your dyes will be captured at the same channel, but one after the other.
Keep in mind that there is more time-delay between imaging your 2 colours compared to the simultanous or line sequential mode, which is normally used.
During live mode I would recommand to only use one channel at a time as it takes more time to see the effect in the second channel. This can be easily done in the software by activating-deactivating a channel (also explained in the ppt in attach).
More information in attach and also in the help folder, including a picture with settings allowing to reload the settings for GFP - PI FRAME sequential imaging:
S:\research\images\images_cameras\confocal\Help Zeiss Exciter\20191108_Frame sequential scanning GFP PI\GFP and PI_Frame sequential.lsm
Don't hesitate to contact Daniel or me if you would have problems with the lightpath setup.
I will keep you update about the repair of this channel.
Gr,
Evelien
Evelien Mylle - Labmanager/ microscopy technician
Advanced Live Cell Imaging
Advanced Live Cell Imaging
VIB-UGent Center for Plant Systems Biology
Ghent University
Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium
Tel. +32(0)9 331 39 74
www.psb.ugent.be
Genome editing, cutting-edge technology for a sustainable agriculture
