Dear olympus confocal users,

The argon laser is giving up its last forces: at the moment there is only a very minimal output, hardly impossible to image something. 

The malfunctioning 515 laser, the difficult start up of the argon laser were the first signs of decay.
The laser has been ordered for a while, but for now there is no news when it will be delivered and placed. 

So now is the ideal time for imaging with the 405 and 559 laser.

I will keep you further informed.

Greetings,

Evelien




From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be>
To: "confocal" <Confocal@psb.vib-ugent.be>, "Ignacio ACHON" <igach@psb.vib-ugent.be>
Sent: Friday, October 30, 2020 4:39:44 PM
Subject: [Confocal] olympus confocal: IMPORTANT!! switching off adapted        procedure!!!
Dear Olympus users,
@ Ignachio, can you confirm as you are the last user today. (I could not find you in the lab)

Please follow the instructions in picture below or in attach in order to switch ONLY the 559 and fluorescence lamp off, so LEAVING THE ARGON LASER ON!! 

Reason is the following: 
The argon laser took this morning more than one hour to get properly working.   
THis, together with the non -working 515 laser, makes me thinks that the argon laser is at his end.  
From experience, not getting the laser on is often one of the first signs.  Once it's on, it's all OK. 
That's why I adjust the switching off procedure by leaving the laser on!  It is a bit risky, with a possible lockdown in prospect, but I don't want to take the risk of not being able to activate the laser on Monday.  The reservations are full for next week, and possibly these are experiments that will be finalized next week.

Do switch off the 559 and the  fluorescence lamp!  
See cadaped switching off procedure below: 


Gr,
Evelien


Gr,

Evelien

Evelien Mylle - Labmanager/ microscopy technician
Advanced Live Cell Imaging

VIB-UGent Center for Plant Systems Biology
Ghent University
Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium
Tel. +32(0)9 331 39 74
www.psb.ugent.be

Genome editing, cutting-edge technology for a sustainable agriculture


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