Dear all, The problems with visualizing your sample through the microscope on the Leica SP8 got worse. We now also have the situation that we have no light at all at the microscope. Switching between live view (in the LAS X software), and/or by switching the lens, and/or switching between DIC and POL light (microscope setting), and the shutters sometimes helps. Restarting the PC doesn't seem to help much, but you can always try. Company will come. Sorry for this inconvenience, Gr, Evelien
From: "Evelien Mylle" <Evelien.Mylle@psb.vib-ugent.be> To: "confocal" <Confocal@psb.vib-ugent.be> Sent: Friday, August 30, 2019 5:01:07 PM Subject: [Confocal] Leica SP8: sometimes problem with microscope settings
Dear all,
The Leica SP8 gives sometimes problems when looking through the microscope to your sample: there is less light and you don't get your sample nicely in focus. It can be solved by clicking (several times) on live view (in the LAS X software), and/or by switching the lens, and/or switching between DIC and POL light (microscope setting). Closing and restarting the PC sometimes helps, but not always, and it is very time consuming. So, I would go for the above options. I assume that there is some kind of filter that gets blocked.
Keep this in mind when doing your imaging especially if you are doing treatments, as it could take a bit more time to set up your imaging.
I will keep you updated.
Gr, Evelien
Evelien Mylle - Labmanager/ microscopy technician Advanced Live Cell Imaging
VIB-UGent Center for Plant Systems Biology Ghent University Technologiepark-Zwijnaarde 71 - 9052 Ghent - Belgium Tel. +32(0)9 331 39 74 [ http://www.psb.ugent.be/ | www.psb.ugent.be ]
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