From: "Evelien Mylle" <evmyl@psb.vib-ugent.be>
To: "confocal" <Confocal@psb.vib-ugent.be>
Sent: Tuesday, March 13, 2018 12:46:03 PM
Subject: [Confocal] Leica Sp2: 514 laser is unstable, sometimes it works, sometimes not.  Other lasers are fine.

Dear confocal lasers,

The 514 laser from the Leica SP2 gives unstable output.  Sometimes working nicely, other times not.

The other lasers are working nicely.   
Depending on your dye or fluorophore it could be possible to excite your sample by using another laserline.  Leica SP2 has also a 496 laser which is the closest to the 514 laser.   

The company is informed and I will keep you updated.

Best regards,

Evelien

Evelien Mylle - Lab Manager / Technician
Advanced Live Cell Imaging

VIB-UGent Center for Plant Systems Biology
Ghent University
Technologiepark 927 - 9052 Ghent - Belgium
Tel. +32(0)9 331 39 34
www.psb.ugent.be

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